The present study was carried out with six wheat and four maize genotypes from Mahyco Research Centre, Dawalwadi, Jalna, MH to develop wheat double haploid lines by wheat x maize crossing system. The following steps were followed while conducting the experiment: emasculation of wheat spikes, crossing or pollination of emasculated spikes with maize pollens, treating pollinated spikes with 100 ppm 2,4-D solution, excision of embryo and subsequent culture on half strength MS media. In total, 296 spikes were emasculated and pollinated with randomly collected maize pollens after 24 hrs. Success was obtained in rescuing a total of 27 embryos from 575 caryopses among two genotypes i.e. MW2 and MW3. Percentage of embryo formation varied in the range of 0-10 per cent owing to non-standard conditions. However, the frequency of embryo formation could be increased by focusing on the most important of all culture conditions i.e. temperature (18-21 °C).
Anjanabha Bhattacharya, Bhavesh Palan, Bharat Char. An insight into wheat haploid production using wheat x maize wide hybridization. J App Biol Biotech. 2015; 3 (05): 044-047. DOI: 10.7324/JABB.2015.3509
1. Grauda D, Lepse N, Strazdina V, Kokina I, Lapina L, Mikelsone A, Lubinskis L, Rashal I. Obtaining of doubled haploid lines by anther culture method for the Latvian wheat breeding. Agronomy Research (Special Issue III). 2010; 8: 545-552.
2. Lashermes P. Improved anther culture method for obtaining direct regeneration in wheat (Triticum aestivum L.). Journal of Genetics and Breeding. 1992; 46: 99-102.
3. Saeed NA, Chowdhry MA, Khan IA. Induction of callus and organogenesis in bread wheat through anther culture. Pakistan Journal of Agriculture Research. 1994; 15(1): 108-114.
4. Lantos C, Paricsi S, Zofajova A, Weyen J, Pauk J. Isolated microspore culture of wheat (Triticum aestivum L.) with Hungarian cultivars. Acta Biologica Szegediensis. 2006; 50(1-2): 31-35.
5. Liu W, Zheng MY, Konzac CF. Improving green plant production via isolated microspore culture in bread wheat (Triticum aestivum L.). Plant Cell Report. 2002; 20: 821-824.
6. Shahinul Islam SM. The effect of colchicine pretreatment on isolated microspore culture of wheat (Triticum aestivum L.). Australian Journal of Crop Science. 2010; 4(9): 660-665.
7. Zheng MY. Microspore culture in wheat (Triticum aestivum)-doubled haploid production via induced embryogenesis. Plant Cell, Tissue and Organ Culture. 2003; 73: 213-230.
8. Campbell AW. Assessment of wheat x maize doubled haploid technology for genetic improvement of New Zealand wheat. A Ph.D. thesis submitted to Lincoln University, Canterbury, New Zealand. 1999.
9. Khan MA, Ahmad J. In vitro wheat haploid embryo production by wheat x maize cross system under different environmental conditions. Pakistan Journal of Agriculture Sciences. 2011; 48(1): 49-53.
10. Niroula RK, Bimb HP, Thapa DB, Sah BP, Nayak S. Production of haploid wheat plants from wheat (Triticum aestivum L.) x maize (Zea mays L.) cross system. Himalayan Journal of Sciences. 2007; 4(6): 65-69.
11. Inagaki M, Mujeeb-Kazi A. Comparison of polyhaploid production frequencies in crosses of hexaploid wheat with maize, pearl millet and sorghum. Breeding Science. 1995; 45: 157-161.
12. Inagaki MN, Tahir M. Production of haploid wheat through intergeneric crosses. Hereditas. 1992; 116: 117-120.
13. Garcia-Llamas C, Ramirez MC, Ballesteros J. Effect of pollinator on haploid production in durum wheat crossed with maize and pearl millet. Plant Breeding. 2004; 123: 201-203.
14. Laurie DA, Bennett MD. Wheat x maize hybridization. Canadian Journal of Genetics and Cytology. 1986; 28: 313-316.
15. Moradi P, Haghnazari A, Bozorgipour R, Sharma B. Development of yellow rust resistant doubled haploid lines of wheat through wheat x maize crosses. International Journal of Plant Production. 2009; 3(3): 77-88.
16. Khan MA, Shaukat S, Ahmad J, Kashif M, Khan AS, Iqbal MZ. Use of intergeneric cross for production of doubled haploid wheat (Triticum aestivum L.). Journal of Science, Technology and Development. 2012; 31(4): 295-300.
17. Niroula RK, Bimb HP. Overview of wheat x maize system of crosses for dihaploid induction in wheat. World Applied Sciences Journal. 2009; 7(8): 1037-1045.
18. Laurie DA, Bennett MD. The production of haploid wheat plants from wheat x maize crosses. Theoretical and Applied Genetics. 1988; 76: 393-397.
19. Ahmad J, Chowdhry MA. The efficiency of chromosome doubling in haploids derived from wheat x maize crossing. Pakistan Journal of Biological Science. 2005; 8(12): 1707-1711.
20. Niroula RK, Thapa DB. Response of Wheat Genotypes to Maize Mediated Polyhaploid Production. American-Eurasian Journal of Agronomy. 2009; 2 (3): 156-161.
21. Torres LE, Bima P, Maich R, Badiali OJJ, Nisi J. Production of haploid plants from ten hybrids of bread wheat (Triticum aestivum L.) through wide hybridization with maize (Zea mays L.). Agriscientia. 2010; Vol. XXVII (2): 79-85.
Year
Month
Agronomic evaluation of temperate maize populations in tropical zone
Justin AbadassiBioprospecting of endophytic bacteria from the Indian Himalayas and their role in plant growth promotion of maize (Zea mays L.)
Kusam Lata Rana, Divjot Kour, Tanvir Kaur, Rubee Devi, Ashok Yadav, Ajar Nath YadavUpsurge production of cellulase from maize stover under soildstate conditions mediated by Streptomyces enissocaesilis DQ026641
Ashok Sudarshan, Siddanna Renuka, Sirasagar Reshma, Bhalerao Shilanjali, Dayanand AgsarAccumulation of cadmium in maize roots inoculated with root organ culture of Rhizophagus irregularis improving cadmium tolerance through activation of antioxidative defense enzymes
Rajamony VinuRadha, Karunanandham Kumutha, Asish Kanagaraj BinodhEffect of growth regulators on callus morphology of Rice anther culturec
Avinash Sharma, Dalpat Lal and Monoj SutradharExploring factors affecting anther culture in rice (Oryza sativa L.)
Swapan Kumar Tripathy, D. Swain, P. M. Mohapatra, Arjun M. Prusti, Bandita Sahoo, Sucharita Panda, Monalisha Dash, Bhaskar Chakma, Suraj K. BeheraFrequencies of Insulin- Promoter Factor-I (IPF-I) gene mutations in a Cohort of Sudanese Patients with Type 2 Diabetes Mellitus
Nahla A Elsheikh and Eltahir AG KhalilMeta-analysis of variant rs 7903146 of TCF7L2 gene with secondary complications of Type 2 Diabetes Mellitus
Kanhaiya Singh, NK Agrawal, SK Gupta, Kiran SinghHumoral immune response in cattle and buffaloes vaccinated against Pasteurella multocida B: 2 with reference to levels of B Lymphocytes and IL-2R+
Harsh Shaveta, Deepti Chachra, H M Saxena, Mudit ChandraComparative analysis of two catalytically distinct endoglucanases from Aspergillus nidulans
Baljit Kaur, H.S. Oberoi, B.S. ChadhaInsight into interplay of some molecular chaperones in a hill stream teleost, Barilius bendelisis under wide seasonal and diurnal thermal fluctuations
Saurabh Dewan,Yashpal Singh,JP BhattCloning, expression and purification of resistance gene analogue RGPM 301 from pearl millet in Escherichia coli
Mariswamy Veena, Prasad Melvin, Sekhar Shailasree, Kukkundoor Ramachandra KiniGrowth, physiological and biochemical responses of Meliaceae species - Azadirachta indica and Melia dubia to elevated CO2 concentrations
S. Janani, P. Priyadharshini, R. S. C. Jayaraj, C. Buvaneswaran, Rekha R. WarrierEffects of Malaria in Pregnancy (MiP) on Pregnancy Development and its Outcome: a Critical Review
Joshua Djabanor, Elvis Quansah, Du-Bois AsanteIn-Vitro Propagation of Dioscorea alata for Tyrosinase Production
B. Gopal Samy, K. Jegatheesan, C. Infant FrancinaIn-vitro Assessment of Carbendazim and Copper oxychloride cytotoxicity on HaCaT and HepG2 human cell lines
Diksha Sateesh Bakre, Basappa Basawanneppa KaliwalAroclor 1254 induced oxidative stress and histopathological changes in mice liver
Jalpa Raja, Shweta Pathak, Rahul KunduCytotoxicity and antiproliferative effects of ethyl acetate fraction of Padina australis against MCM-B2 and K562 cell lines.
Heder Djamaludin, Maria Bintang, Bambang Pontjo PriosoeryantoLow dose and subchronic Aroclor 1254 induced histopathological changes in mice kidney
Jalpa Raja, Shweta Pathak, Rahul KunduStudy of the changes in the growth, protein, and bioactive profile of Chlorella emersonii KJ725233 in response to sodium and ammonium nitrate
Sneha Sunil Sawant, Varsha Kelkar-ManeStudy of impact of Wolbachia surface protein on reactive oxygen species production in HepG2 cell line exposed to ethanol
V. P. Kishore, H. P. PuttarajuMycelial biomass, antioxidant, and myco-actives of mycelia of abalone mushroom Pleurotus cystidiosus in liquid culture
Kent Garcia, Chester Jhae Garcia, Reynante Bustillos, Rich Milton DulayDetection of 3243 A/G and 3316 G/A mitochondrial DNA mutations in Nagpur population
Utpal Jagdish Dongre, Virendra Govindrao MeshramDetection of SARS-CoV-2 using high-throughput PCR
Saakshi Jalali, Vikas K. Patel, Venkatesh Prasad, Ajit Sapre, Santanu Dasgupta, Bhaskar BhadraIn vitro antioxidant activity of Lactobacillus plantarum against hydrogen peroxide-induced neuronal damage on PC12 cells
Shani Kunjamma John, Vani ChandrapragasamA novel strategy for disarming quorum sensing in Pseudomonas aeruginosa-Chlorella emersonii KJ725233
Sneha Sawant Desai, Reema Devi Singh, Sukhendu B Ghosh, Varsha KelkarRecent advances in the knowledge of coronaviruses with special emphasis on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Khushboo Irshad, Madeeha Mudassir, Sheeba Irshad, Mohammad IrshadStatistical optimization of chitinase production by Box–Behnken design in submerged fermentation using Bacillus cereus GS02
Garima Dukariya, Anil KumarEnhancement of Vitamin D2 content through ultraviolet-B irradiation in submerged cultivated Pleurotus eryngii mycelia using response surface methodology
Umesh Singh, Ashwani Gautam, Satyawati SharmaModification of the time of incubation in colorimetric method for accurate determination of the total antioxidants capacity using 2,2-diphenyl-1-picrylhydrazyl stable free radical
Abhipsa Bal, Samar Gourav Pati, Falguni Panda, Biswaranjan PaitalThe linkage between the second wave of COVID-19 and the severity of mucormycosis in India
Kshama Wamanrao Murarkar, Shilpa Prakash MankarAugmentative role of Piriformospora indica fungus and plant growth promoting bacteria in mitigating salinity stress in Trigonella foenum-graecum
Sanskriti Bisht, Shatrupa Singh, Madhulika Singh,, Jai Gopal SharmaThe ITS2 DNA sequence analysis in six species of barbin fishes with phylogenetic insights
Sandip Choudhury, Briyanka Kashyap, Karabi DuttaDiversity due to mutations in circulating virus strains of SARS-CoV-2 may delay control of COVID-19
Sharanagouda S. Patil, Chandan Shivamallu, Chandan Dharmashekara, Sushma Pradeep, Kuralayanapalya Puttahonnappa Suresh, Ashwini Prasad, Shiva Prasad Kollur, Mahendra P. Yadav, Chandrashekar Srinivasa, Bramhadev PattnaikGenetic polymorphism of soybean genotypes with contrasting levels of phosphatidylcholine, protein, and lipoxygenase-2
Aseem Kumar Anshu,, Trupti Tayalkar, Anita Rani, Vineet Kumar, Hamendra Singh ParmarCloning the root-specific Asy promoter and genes encoding chitinase 42 kDa of Trichoderma asperellum into the plant expression vector
Nguyen Hoang Tue, Tran Gia Cat Tuong, Pham Thi Huyen Trang, Nguyen Duc Chung, Phung Thi Bich Hoa,, Nguyen Quang Duc Tien, Nguyen Hoang LocSeed treatment with 24-epibrassinolide enhances soybean seed germination under salinity stress
Victoria Oko Otie, Idorenyin Udo, Shuoshuo Liang, Shao Yang, Michael Itam, Ping An, Egrinya Anthony EnejiCowpea (Vigna unguiculata L. Walp.): A choice crop for sustainability during the climate change periods
Tewodros Ayalew, Tarekegn YosephAntifungal effects of Kurthia gibsonii Mb 126 chitinase as a seed treatment on seed-borne fungi of rice seed on germination percentage and seedling vigor
Mini K. Paul, K. D. Mini, Jyothis MathewHealth-related risk of SARS-CoV-2 infection in chronic obstructive pulmonary disease patients: A systematic review
Meenakshi S, Raghunath N, Vishal B Rawal, Ramith RamuMeta-analysis of Type 2 diabetes and insulin resistance gene expression datasets to decipher their associated pathways
Aditya Saxena, Nitish Mathur, Uma Chaudhary, Utkarsh Raj, Sneha Rai, Sandeep Kumar MathurA subchronic toxicity test of a combination of n-hexane and ethyl acetate fraction of rose myrtle leaves (Rhodomyrtus tomentosa [Ait.] Hassk) on male white rats
Salni Salni, Herlina Herlina, Aprila PurnamasariDesigning of guide RNA constructs for CRISPR/Cas9-mediated editing of rice transcription factor OsMADS26 for enhancing drought tolerance
K. Anjala, Rehna AugustineHumoral immune response in COVID-19 patients and novel design of lateral flow assay strip for simultaneous rapid detection of IgA/IgM/ IgG antibodies against the SARS-CoV-2 virus
Huong Thu Ngo, Son Van Tran, Hien Dang Nguyen, Phong Quoc TruongIsolation and characterization of robust plant growth-promoting rhizobacteria from lignite mines, Gujarat
Ravi Patel, Dilip N. Borada, Amisha Patel, Neil J. ShahIn-vitro and in-vivo models for the identification and validation of radioprotectors and radiosensitizers
Debasish Mohapatra,, Amlan Priyadarshee Mohapatra,, Anjan Kumar Sahoo, Shantibhusan SenapatiBegomovirus detection in the whitefly Bemisia spp. on eggplant Solanum melongena L. leaves
Rachmi Putri, Prasetya Anugerah Gusti, Nastiti WijayantiEvaluation of functional characteristics of roselle seed and its use as a partial replacement of wheat flour in soft bread making
Nguyen Minh Thuy, Nguyen Bao Tram, Dinh Gia Cuong, Huynh Khanh Duy, Ly Thanh An, Vo Quoc Tien, Tran Ngoc Giau, Ngo Van TaiDevelopment of a colloidal Gold nanoparticle-based lateral-flow assay for rapid detection of SARS-CoV-2 showing enhanced sensitivity and specificity
Suresh Singh, Tapan Singha, Rajat Maheshwari, Pankaj Yadav, Arun Kumar, Harendra Bhoj, Bharti Sharma, Amit Verma, Amit Gupta, Harsh Singh, Sonal Mishra, Rajeshwar SinhaEffects of gibberellic acid on seed dormancy of black gram (Vigna mungo L.)
Vinothini Nedunchezhiyan, Mohanasundari Palanivel, P. A. Akhila Jabeen, Poovarasan Thangavel, Bhavyasree Ramakrishnan, Manonmani Velusamy, Sakila Muthusamy, Isong Abasianyanga EdmNeutrophil gelatinase-associated lipocalin a proinflammatory polypeptide necessary for host cell survival in bacterial infection
Nichita Yadav Aare, Pawan Kumar Anoor, Swathi Raju M, N. Srinivas Naik, Sandeepta BurgulaProduction of SARS-CoV-2 nucleocapsid protein in Escherichia coli and its characterization
Vaishali Verma, AbhishekIdentification of matrix metalloproteinases inhibitors from methanol extract of Peltophorum pterocarpum leaves
Bhimrao V. Jaiwal, Faiyaz K. Shaikh, Sarwan W. Bradosty, Ajit B. Patil, Raghavendra L. S. HallurSustainable biodegradation of textile dye reactive blue 222 by the novel strain Enterobacter CU2004, isolated from the industrial waste: A design of experiment based optimization study and characterisation of metabolites
Vasantha Veerappa Lakshmaiah, Anish Nag, Suresh Gotekar, Sunil More, Shobha K. JayannaIsolation and Characterization of Cellulase-Producing Myxobacterial Strain from the Unique Niche of Mirgund Wetland from the North-Western Himalayas
Daljeet Singh Dhanjal, Simranjeet Singh, Vijay Kumar, Praveen C. Ramamurthy, Chirag Chopra, Atif Khurshid Wani, Reena Singh, Joginder SinghA novel trypsin inhibitor peptide MoCh I with antimicrobial activity derived from Momordica charantia
Samriti Dogra, Rajesh Biswas, Rupinderjeet Kaur, Sangeeta Sharma, Kakoli BiswasIsolation of toxic gas-producing bacteria (Desulfovibrio spp.) from shrimp ponds and potential of bacteriophages as biocontrol
Truong Thi Bich Van, Tran Vo Minh ThuNovel use of probiotic as acetylcholine esterase inhibitor and a new strategy for activity optimization as a biotherapeutic agent
Abdulrahman M. Qadah, Amr A. El-Waseif, Heba YehiaEvaluation and comparison of 15 short tandem repeat loci of south and west Indian population for use in personal identification applications
Prabakaran Mathiyazhagan, Thangaraju Palanimuthu, Agasthi PadmanathanEffect of phenolic disinfectant on sporulation inhibition of Eimeria tenella for prevention of coccidiosis
Rajendra Moorthy Rajendran, Nabila Fatima SIn silico screening and analysis of candidate microRNA-target interaction involved in progression of prediabetes to Type II Diabetes Mellitus
Angel Mendonca, Prabu Thandapani, Priya Swaminathan, Sujatha SundaresanSugarcane yield prediction using NOA-based swin transformer model in IoT smart agriculture
V. Gokula Krishnan, B. V. Subba Rao, J. Rajendra Prasad, P. Pushpa, S. KumariRole of DREB genes in the regulation of salt stress-mediated defense responses in plants
Ashokkumar Ramakrishnan Yadav, Vaishnavi Ashokkumar, Suganthi Muthusamy, Senthilkumar PalanisamyRapid and visual detection of leptospira interrogans using polymerase spiral reaction assay
Archana Vishwakarma, Punith Chowdary, Mohandass RamyaPurification and structural characterization of Chojalactone C, a promising anticancer compound from a marine bacterium, Paenibacillus lentimorbus SAGM 3
Nagamuthu Vinothkumar, Pachaiappan PugalendhiCombinatorial network of transcriptional and post-transcriptional regulation in amyotrophic lateral sclerosis
Rupesh Kumar, Pammi Gauba, Shazia HaiderPhytochemicals of Nardostachys jatamansi as potential inhibitors of HCV E2 receptor: An in silico study
Lalrintluanga Hnamte, Praveen Nagella, Aatika Nizam, Vasantha Veerappa LakshmaiahImpact of nanoparticles on microalgae and the prospects for biofuel production: Current advancements and future outlook
Richa Pahariya, Abhishek Chauhan, Anuj Ranjan,, S. K. Thakur¸ Hardeep Singh Tuli, Seema Ramniwas, Moyad Shahwan,, Tanu JindalTriphala extract Reduces Anti-apoptotic Protein (Mcl-1) Expression through the Activation of Caspase 3 in Hepatocellular Carcinoma Cells
Yoganchal Mishra, Rohit Raj Yadav, Kavita Verma, Neelam YadavPhylogenetic analysis of Omicron subvariants in Vietnam
Phuoc Huynh, Huyen Thi Thuong Nguyen, Quan Ke Thai