Research Article | Volume: 5, Issue: 5, Sep-Oct, 2017
Rational Design of Duplex Specific Nuclease for One-Step Isothermal Viral RNA Detection
RNA viruses are a potent human adversary, evidenced by several global pandemics including the Ebolavirus in West Africa, the emerging Zika virus, and outbreaks of new Influenza strains and Norwalk virus in the food supply and cruise ships. Despite the virulence of these pathogens, there remains a significant limitation for detecting these viruses in a fast, accurate and cost effective manner. To meet this need we present a modified form of the duplex specific nuclease enzyme from the Paralithodes camtschaticus crab capable of generating an RNA-based signal amplification in a fraction of the time required for standard RT-qPCR. The applicability of this enzyme is demonstrated in an assay for Norwalk virus detection with a lower limit of ~100 viral copies per liter of environmental water.
Wurtzler EM, Ravi R, Kapoor V, Wendell D. Rational Design of Duplex Specific Nuclease for One-Step Isothermal Viral RNA Detection. J App Biol Biotech. 2017; 5 (05): 1-10.
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Balachander Durairaj, Sudheesh K. Prabhudas, Jaiganesh Rengarajan, Iyappan SellamuthuMolecular identification of endophytic fungi associated with Coleus forskohlii (Willd.) Briq.
Grace Leena Crasta,, Koteshwar Anandrao Raveesha,Augmentative role of Piriformospora indica fungus and plant growth promoting bacteria in mitigating salinity stress in Trigonella foenum-graecum
Sanskriti Bisht, Shatrupa Singh, Madhulika Singh,, Jai Gopal SharmaExpression of FUB-1 and FUB-11 as Toxic genes responsible for virulence during pathogenesis and combination of biocontrol agents in inhibition of Fusaric acid of Fusarium oxysporum causing Fusarium wilt of Arachis hypogaea L.
Pilli RajeswariDiversity due to mutations in circulating virus strains of SARS-CoV-2 may delay control of COVID-19
Sharanagouda S. Patil, Chandan Shivamallu, Chandan Dharmashekara, Sushma Pradeep, Kuralayanapalya Puttahonnappa Suresh, Ashwini Prasad, Shiva Prasad Kollur, Mahendra P. Yadav, Chandrashekar Srinivasa, Bramhadev PattnaikRole of microRNAs in the progression and metastasis of gastric cancer
Havisha Dinesh, Megala JayaramanIdentification, production, and purification of a novel lipase from Bacillus safensis
Krishna Patel, Samir ParikhIsolation and characterization of starch degrading bacteria from disparate soil samples
Vidhyutha Srivathsan, Mahima Bhandari, Priya SwaminathanExosome mediated cell signal toward breast cancer metastasis: A comprehensive review
Manikantan Pappuswamy, Anushka ShitutDesigning of guide RNA constructs for CRISPR/Cas9-mediated editing of rice transcription factor OsMADS26 for enhancing drought tolerance
K. Anjala, Rehna AugustineTotal phenolic, flavonoid contents, and antioxidant activity of three selected Portulaca grandiflora mutants in MV8 generation as a result of recurrent irradiation technique
Waras Nurcholis,, Syarifah Iis Aisyah, Regina Agritena Mayrischa Saraswati, Yoshua Shandy YudhaIsolation and characterization of keratinolytic bacteria from poultry waste soils of Himachal Pradesh
Richa Vema, Vijay KumarThe physicochemical and biological properties of novel silver nanoparticles synthesized by the extract of Holigarna ferruginea
Kumbar Mudakappa Manjunath, Y. L. KrishnamurthyIsolation and characterization of bacteria possessing Osmotolerance activity from phylloplane of Centella asiatica
Sonal Gupta, Ashwini A. WaooA metagenomic analysis of gut microbiome phylogeny among four economically important carp species from wild and aquaculture farms
Shrihari Ashok Pingle, Abhay John KhandagleImmune cells at the maternal-fetal interphase: Role in implantation and establishment of tolerance
Anju KaickermiR-122 and miR-21 as clinical biomarkers in hepatocellular carcinoma: A review
Nishant Kumar, Priyvart Choudhary, Narotam Sharma, Alok Tripathi, Nishesh SharmaIsolation of toxic gas-producing bacteria (Desulfovibrio spp.) from shrimp ponds and potential of bacteriophages as biocontrol
Truong Thi Bich Van, Tran Vo Minh ThuIn silico screening and analysis of candidate microRNA-target interaction involved in progression of prediabetes to Type II Diabetes Mellitus
Angel Mendonca, Prabu Thandapani, Priya Swaminathan, Sujatha SundaresanAssessment of biodegradation potential of lead-resistant bacteria isolated from polluted sites of Gomati River in Lucknow
Afreen Shahid, Farhan Ahmad, Chitranshu Pandey, Sunil Kumar, Aisha KamalCombinatorial network of transcriptional and post-transcriptional regulation in amyotrophic lateral sclerosis
Rupesh Kumar, Pammi Gauba, Shazia HaiderEvaluation of pancreatic lipase and angiotensin-converting enzyme inhibitors from ethanolic extract of butterfly pea (Clitoria ternatea L.) flowers
Ratna Djamil, Diah Kartika Pratami, Tia Novia Wilantika, Qurrata A’yun, Abdul Mun’im, Asep Bayu, Masteria Yunovilsa PutraIn silico design of sgRNA for CRISPR/Cas9-mediated FaRALF33 gene mutagenesis to decrease the infection process to Colletotrichum acutatum in strawberry
Angel David Hernández-Amasifuen,, Mao Yupanqui-Celestino, Alexandra Jherina Pineda-Lázaro, Elvin Delgado-Mera, Linder Ramírez-Viena, Carlos Roberto Pesantes-Rojas, Mike Anderson Corazon-GuivinAZIN1-centric dynamic network biomarker of tipping point in non-small cell lung cancer metastasis
Anita Chauhan, Kuntal Kalra, Seema KalraIdentification of differential expressed genes and its related pathways in Hela cell line treated with urea noscapine as potent anticancer agent
Animesh Pattnaik, Pradeep Kumar NaikPhylogenetic study of some major Dendrobium species of Eastern Himalaya using internal transcribed spacer marker
Animesh Mondal, Kalyan Kumar DeAnticancer effect of bioactive compound Apparicine isolated from the Tabernaemontana divaricata on retinoblastoma cancer cell line (Y79) and in silico docking approaches
Elango Rajeswari, Balu Prakash, Durai Mahendran, Devarajan NatarajanSensitive and cost-effective citrate-based RNA extraction procedure for isolation of RNA from Tilapia Lake Virus-infected fish
S.R. Saranya, R. SudhakaranEnhanced quinalphos degradation using immobilized cells of Enterobacter cloacae isolated from farm land soil of Vazhayur Panchayath, Malappuram District, Kerala
Nhattuketty Krishnan Shainy, Asha Bhakthavalsalam, Shabanamol Subaidabeevi, Farha Baramy, Aswathi Ayiliath, Kulandhaivel MurugesanA simple approach to generate transgenic cell lines with high efficiency using CRISPR and flippase-mediated recombination
Sudipta Sarma, Rohan Kamat, Shiny C. ThomasIdentification and pathogenicity assessment of bacterial strain P1 isolated from the gut of diseased Eri silkworm, Samia ricini Donovan
Dimpi Moni Kalita, Sunayan Bardoloi, Sanghamitra Saharia, Shibani Kalita, Sanjana Sharmin, Dhirunabh Swargiary, Tapashi Sutradhar, Kakali DekaAntifungal activity of Bacillus sp. against Colletotrichum causing anthracnose in postharvest banana fruits
Thi Cao Van Quach, Huyen Thanh Thi Vo, Truc Trung NguyenIsolation and molecular analysis of bacilli producing bacteriocins with broad-spectrum antimicrobial potential from fermented milk and kefir
Namita Singh, Anand Narayan Singh, Minhaj Ahmad KhanTerfezia claveryi truffles at Hafr Al Batin, Saudi Arabia: A study based on morphological and molecular characterizations
Sabry Younis MahmoudIn silico analyses to identify the potential genes and microRNA targets in pathways mediating radiation-induced adaptive response in breast cancer
Aishwarya Thathamangalam Ananthanarayanan, Rajnish Narayanan, Satish Srinivas Kondaveeti, Ilangovan Ramachandran, Tamizh Selvan Gnanasekaran, Thirumurthy Madhavan, Venkatachalam PerumalIn vitro micropropagation and flowering of the endangered medicinal herb Iphigenia stellata Blatt.
Gayatri Vaze, Pramod Hurkadale, Harsha Hegde