Bioremediation of hazardous azo dye methyl red by a newly isolated Enterobacter asburiae strain JCM6051 from industrial effluent of Uttarakhand regions

Swati; Padma Singh

doi:10.7324/JABB.2022.10s206

Abstract References Article Metrics Similar Articles Request Permission Related Search Citation Alert By Google Scholar Comment On This Article

Abstract

Azo dyes are often known to be carcinogenic mutagenic and recalcitrant. Dyeing effluents have emerged as a significant cause of water contamination. Dyes influence all living forms, included humans, due to their xenobiotic characteristics and toxicity, as a result, hazardous dyes from colored wastewater must be treated and removed before they are released into the ecosystem. Bioremediation is an innovative, cost-effective, and eco-friendly achievement of biotechnological novelty. Thirty dye-decolorizing indigenous strains were isolated from industrial wastewater in the present investigation from the Kashipur paper industry and SIDCUL industrial area Haridwar using nutrient broth medium amended with 100 mg/l methyl red (MR). Isolation of MR decolorizing bacteria was done by the serial dilution method followed by the spread plate method. A total of 30 isolates were isolated and subjected to primary screening which was done through the tube method. Following a primary screening, 10 potent strains were retained for further evaluation of the efficacy of color removal, designated as MRD2, MRD3, MRD4, MRD15, MRD17, MRD18, MRD19, MRD20, MRD22, and MRD28, which were presumably grouped into 10 genera according to morphology and biochemical assay. The bacterial strain MRD17 outperformed other tested strains via a decolorization assay with 74.28% degradation and decolorization of MR in 72 hours, which was further, identified as Enterobacter asburiae strain JCM6051 by 16S rRNA sequencing and submitted to the NCBI GenBank with accession number MT539179. In addition, the thermodynamic stability of the strain’s 16S rRNA sequence was investigated using bioinformatics tools such as mfold and NEB cutter. These findings suggest that bacterial isolates might be useful in the development of an alternative and environmentally acceptable approach for decolorizing and degrading azo dyes from industrial waste.

Keyword: Bioremediation decolorization recalcitrant Enterobacter asburiae methyl red

Citation:

Swati, Singh P. Bioremediation of hazardous azo dye methyl red by a newly isolated Enterobacter asburiae strain JCM6051 from industrial effluent of Uttarakhand regions. J Appl Biol Biotech 2022;10(Suppl 2):64–72.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

Reference

1. Shamraiz U, Hussain RA, Badshah A, Raza B, Saba S. Functional metal sulfides and selenides for the removal of hazardous dyes from Water. Photochem Photobiol 2016;159:33-41.
https://doi.org/10.1016/j.jphotobiol.2016.03.013
2. Amin S, Rastogi RP, Chaubey MG, Jain K, Divecha J, Desai C, et al. Degradation and toxicity analysis of a reactive textile diazo dye- Direct Red 81 by newly isolated Bacillus sp. DMS2. Front Microbiol 2020;11:1-12.
https://doi.org/10.3389/fmicb.2020.576680
3. Saratale RG, Saratale GD, Chang JS, Govindwar SP. Bacterial decolorization and degradation of azo dyes: a review. J Taiwan Inst Chem Eng 2011;42:138-57.
https://doi.org/10.1016/j.jtice.2010.06.006
4. Guadie A, Tizazu S, Melese M, Guo W, Ngo HH, Xia S. Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake. Biotechnol Rep 2017;15:92-100.
https://doi.org/10.1016/j.btre.2017.06.007
5. Sarkar S, Ponce NT, Banerjee A, Bandopadhyay R, Rajendran S, Lichtfouse E. Green polymeric nanomaterials for the photocatalytic degradation of dyes: a review. Environ Chem Lett 2020;14:1-12.
https://doi.org/10.1007/s10311-020-01021-w
6. Chen SL, Huang XJ, Xu ZK. Functionalization of cellulose nanofiber mats with phthalocyanine for decoloration of reactive dye wastewater. Cellulose 2011;18:1295-303.
https://doi.org/10.1007/s10570-011-9572-5
7. Prabhakar Y, Gupta A, Kaushik A. Enhanced decolorization of reactive violet dye 1 by halo-alkaliphilic Nesterenkonia strain: process optimization, short acclimatization and reusability analysis in batch cycles. Process Saf Environ Prot 2019;131:116-26.
https://doi.org/10.1016/j.psep.2019.09.004
8. Roy DC, Biswas SK, Sheam MM, Hasan MR, Saha AK, Roy AK, et al. Bioremediation of malachite green dye by two bacterial strains isolated from textile effluents. Curr Res Microb Sci 2020;1:37-43.
https://doi.org/10.1016/j.crmicr.2020.06.001
9. Du LN, Li G, Zhao YH, Xu HK, Wang Y, Zhou Y, et al. Efficient metabolism of the azo dye methyl orange by Aeromonas sp. strain DH- 6: characteristics and partial mechanism. Int Biodeterior Biodegrad 2015;105:66-72.
https://doi.org/10.1016/j.ibiod.2015.08.019
10. Haque MM, Haque MA, Mosharaf MK, Marcus PK. Decolorization, degradation and detoxification of carcinogenic sulfonated azo dye methyl orange by newly developed biofilm consortia. Saudi J Biol Sci 2021;28:793-804.
https://doi.org/10.1016/j.sjbs.2020.11.012
11. Barathi S, Aruljothi K, Karthik C, Padikasan I, Ashokkumar V. Biofilm mediated decolorization and degradation of reactive red 170 dye by the bacterial consortium isolated from the dyeing industry wastewater sediments. Chemosphere 2022;286:131914.
https://doi.org/10.1016/j.chemosphere.2021.131914
12. Zhang F, Yediler A, Liang X, Kettrup A. Effects of dye additives on the ozonation process and oxidation by-products: a comparative study using hydrolyzed CI reactive red 120. Dyes Pigm 2004;60:1-7.
https://doi.org/10.1016/S0143-7208(03)00111-6
13. Yadav A, Mukherji S, Garg A. Removal of chemical oxygen demand and color from simulated textile wastewater using a combination of chemical/physicochemical processes. Ind Eng Chem Res 2013;52:10063-71.
https://doi.org/10.1021/ie400855b
14. John J, Dineshram R, Hemalatha KR, Dhassiah MP, Gopal D, Kumar A. Bio-decolorization of synthetic dyes by a halophilic bacterium Salinivibrio sp. Front Microbiol 2020;11:1-9.
https://doi.org/10.3389/fmicb.2020.594011
15. Sabaruddin MF, Nor MH, Mubarak MF, Rashid NA, Far CG, Youichi S, et al. Biodecolourisation of acid red 27 dye by Citrobacter freundii A1 and Enterococcus casseliflavus C1 bacterial consortium. Mal J Fund Appl Sci 2018;14(2):202-7.
https://doi.org/10.11113/mjfas.v14n2.961
16. Dong H, Guo T, Zhang W, Ying H, Wang P, Wang Y, et al. Biochemical characterization of a novel azoreductase from Streptomyces sp. Application in eco-friendly decolorization of azo dye wastewater. Int J Biol Macromol 2019;140:1037-46.
https://doi.org/10.1016/j.ijbiomac.2019.08.196
17. Zhuang M, Sanganyado E, Zhang X, Xu L, Zhu J, Liu W, et al. Azo dye degrading bacteria tolerant to extreme conditions inhabit nearshore ecosystems: optimization and degradation pathways. J Environ Manage 2020;261:110222.
https://doi.org/10.1016/j.jenvman.2020.110222
18. Telke AA, Ghodake GS, Kalyani DC, Dhanve RS, Govindwar SP. Biochemical characteristics of a textile dye degrading extracellular laccase from a Bacillus sp. ADR. Bioresour Technol 2011;102:1752-6.
https://doi.org/10.1016/j.biortech.2010.08.086
19. Shah PD, Dave SR, Rao MS. Enzymatic degradation of textile dye Reactive Orange 13 by newly isolated bacterial strain Alcaligenes faecalis PMS-1. Int Biodeterior Biodegrad 2012;69:41-50.
https://doi.org/10.1016/j.ibiod.2012.01.002
20. Mohanty SS, Kumar A. Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes. Sci Rep 2021;11:1-15.
https://doi.org/10.1038/s41598-021-87227-6
21. Zong M, Song D, Zhang X, Huang X, Lu X, Rosso KM. Facet-dependent photodegradation of methylene blue by hematite nanoplates in visible light. Environ Sci Technol 2020;55:677-88.
https://doi.org/10.1021/acs.est.0c05592
22. Brito CN, Ferreira MB, de Moura Santos EC, Léon JJ, Ganiyu SO, Martínez-Huitle CA. Electrochemical degradation of Azo-dye Acid Violet 7 using BDD anode: effect of flow reactor configuration on cell hydrodynamics and dye removal efficiency. J Appl Electrochem 2018;48:1321-30.
https://doi.org/10.1007/s10800-018-1257-4
23. Holt JG, Krieg NR, Sneath PH, Staley JT, Williams ST. Bergey's manual of determinative bacteriology. 9th edition, Williams &Wilkins, Baltimore, MD, p 566, 1994.
24. Das D, Banerjee D, Mondal M, Shett A, Das B, Das NS, et al. Nickel doped graphitic carbon nitride nanosheets and its application for dye degradation by chemical catalysis. Mater Res Bull 2018;101:291-304.
https://doi.org/10.1016/j.materresbull.2018.02.004
25. Ganesan P, Reegan AD, David RH, Gandhi MR, Paulraj MG, Al-Dhabi NA, et al. Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India. Alexandria J Med 2017;53:101-10.
https://doi.org/10.1016/j.ajme.2016.03.004
26. Zuker M. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 2003;31:3406-15.
https://doi.org/10.1093/nar/gkg595
27. Karim ME, Dhar K, Hossain MT. Decolorization of textile reactive dyes by bacterial monoculture and consortium screened from textile dyeing effluent. J Genet Eng Biotechnol 2018;16:375-80.
https://doi.org/10.1016/j.jgeb.2018.02.005
28. Balapure KH, Jain K, Chattaraj S, Bhatt NS, Madamwar D. Co-metabolic degradation of diazo dye-reactive blue 160 by enriched mixed cultures BDN. J Hazard Mater 2014;279:85-95.
https://doi.org/10.1016/j.jhazmat.2014.06.057
29. Das S, Bandi V, Arora HS, Veligatla M, Garrison S, D'Souza F, et al. Synergistic catalytic effect of iron metallic glass particles in direct blue dye degradation. J Hazard Mater 2015;30:1121-7.
https://doi.org/10.1557/jmr.2015.90
30. Mahmood R, Sharif F, Ali S, Hayyat MU. Enhancing the decolorizing and degradation ability of bacterial consortium isolated from textile effluent affected area and its application on seed germination. Sci World J 2015;2015:1-9.
https://doi.org/10.1155/2015/628195
31. Ajaz M, Elahi A, Rehman A. Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater. J Water Reuse Desalin 2018;8:507-15.
https://doi.org/10.2166/wrd.2018.065
32. Meerbergen K, Willems KA, Dewil R, Van Impe J, Appels L, Lievens B. Isolation and screening of bacterial isolates from wastewater treatment plants to decolorize azo dyes. J Biosci Bioeng 2018;125:448-56.
https://doi.org/10.1016/j.jbiosc.2017.11.008
33. Ahmed I, Haque F, Rahman MA, Parvez MA, Mou TJ. Screening of methyl red degrading bacteria isolated from textile effluents of Savar Area, Dhaka, Bangladesh. Adv Biosci Biotechnol 2020;11:301-18.
https://doi.org/10.4236/abb.2020.117022
34. Tiar A, Askarne L, Ait addi E, Assabbane A, and Boubaker H. Decolorization of the azo dye methyl red by an isolated bacterium Enterobacter hormaechei strain CUIZ1. J Mater Environ Sci 2018;9:2822-30.

Article Metrics

31 Views 70 Downloads 101 Total

Year

Month

Related Search

By author names

Swati [PubMed] [Google Scholar ]

Singh P [PubMed] [Google Scholar ]

By article title

1. Shamraiz U, Hussain RA, Badshah A, Raza B, Saba S. Functional metal sulfides and selenides for the removal of hazardous dyes from Water. Photochem Photobiol 2016;159:33-41. https://doi.org/10.1016/j.jphotobiol.2016.03.013

2. Amin S, Rastogi RP, Chaubey MG, Jain K, Divecha J, Desai C, et al. Degradation and toxicity analysis of a reactive textile diazo dye- Direct Red 81 by newly isolated Bacillus sp. DMS2. Front Microbiol 2020;11:1-12. https://doi.org/10.3389/fmicb.2020.576680

3. Saratale RG, Saratale GD, Chang JS, Govindwar SP. Bacterial decolorization and degradation of azo dyes: a review. J Taiwan Inst Chem Eng 2011;42:138-57. https://doi.org/10.1016/j.jtice.2010.06.006

4. Guadie A, Tizazu S, Melese M, Guo W, Ngo HH, Xia S. Biodecolorization of textile azo dye using Bacillus sp. strain CH12 isolated from alkaline lake. Biotechnol Rep 2017;15:92-100. https://doi.org/10.1016/j.btre.2017.06.007

5. Sarkar S, Ponce NT, Banerjee A, Bandopadhyay R, Rajendran S, Lichtfouse E. Green polymeric nanomaterials for the photocatalytic degradation of dyes: a review. Environ Chem Lett 2020;14:1-12. https://doi.org/10.1007/s10311-020-01021-w

6. Chen SL, Huang XJ, Xu ZK. Functionalization of cellulose nanofiber mats with phthalocyanine for decoloration of reactive dye wastewater. Cellulose 2011;18:1295-303. https://doi.org/10.1007/s10570-011-9572-5

7. Prabhakar Y, Gupta A, Kaushik A. Enhanced decolorization of reactive violet dye 1 by halo-alkaliphilic Nesterenkonia strain: process optimization, short acclimatization and reusability analysis in batch cycles. Process Saf Environ Prot 2019;131:116-26. https://doi.org/10.1016/j.psep.2019.09.004

8. Roy DC, Biswas SK, Sheam MM, Hasan MR, Saha AK, Roy AK, et al. Bioremediation of malachite green dye by two bacterial strains isolated from textile effluents. Curr Res Microb Sci 2020;1:37-43. https://doi.org/10.1016/j.crmicr.2020.06.001

9. Du LN, Li G, Zhao YH, Xu HK, Wang Y, Zhou Y, et al. Efficient metabolism of the azo dye methyl orange by Aeromonas sp. strain DH- 6: characteristics and partial mechanism. Int Biodeterior Biodegrad 2015;105:66-72. https://doi.org/10.1016/j.ibiod.2015.08.019

10. Haque MM, Haque MA, Mosharaf MK, Marcus PK. Decolorization, degradation and detoxification of carcinogenic sulfonated azo dye methyl orange by newly developed biofilm consortia. Saudi J Biol Sci 2021;28:793-804. https://doi.org/10.1016/j.sjbs.2020.11.012

11. Barathi S, Aruljothi K, Karthik C, Padikasan I, Ashokkumar V. Biofilm mediated decolorization and degradation of reactive red 170 dye by the bacterial consortium isolated from the dyeing industry wastewater sediments. Chemosphere 2022;286:131914. https://doi.org/10.1016/j.chemosphere.2021.131914

12. Zhang F, Yediler A, Liang X, Kettrup A. Effects of dye additives on the ozonation process and oxidation by-products: a comparative study using hydrolyzed CI reactive red 120. Dyes Pigm 2004;60:1-7. https://doi.org/10.1016/S0143-7208(03)00111-6

13. Yadav A, Mukherji S, Garg A. Removal of chemical oxygen demand and color from simulated textile wastewater using a combination of chemical/physicochemical processes. Ind Eng Chem Res 2013;52:10063-71. https://doi.org/10.1021/ie400855b

14. John J, Dineshram R, Hemalatha KR, Dhassiah MP, Gopal D, Kumar A. Bio-decolorization of synthetic dyes by a halophilic bacterium Salinivibrio sp. Front Microbiol 2020;11:1-9. https://doi.org/10.3389/fmicb.2020.594011

15. Sabaruddin MF, Nor MH, Mubarak MF, Rashid NA, Far CG, Youichi S, et al. Biodecolourisation of acid red 27 dye by Citrobacter freundii A1 and Enterococcus casseliflavus C1 bacterial consortium. Mal J Fund Appl Sci 2018;14(2):202-7. https://doi.org/10.11113/mjfas.v14n2.961

16. Dong H, Guo T, Zhang W, Ying H, Wang P, Wang Y, et al. Biochemical characterization of a novel azoreductase from Streptomyces sp. Application in eco-friendly decolorization of azo dye wastewater. Int J Biol Macromol 2019;140:1037-46. https://doi.org/10.1016/j.ijbiomac.2019.08.196

17. Zhuang M, Sanganyado E, Zhang X, Xu L, Zhu J, Liu W, et al. Azo dye degrading bacteria tolerant to extreme conditions inhabit nearshore ecosystems: optimization and degradation pathways. J Environ Manage 2020;261:110222. https://doi.org/10.1016/j.jenvman.2020.110222

18. Telke AA, Ghodake GS, Kalyani DC, Dhanve RS, Govindwar SP. Biochemical characteristics of a textile dye degrading extracellular laccase from a Bacillus sp. ADR. Bioresour Technol 2011;102:1752-6. https://doi.org/10.1016/j.biortech.2010.08.086

19. Shah PD, Dave SR, Rao MS. Enzymatic degradation of textile dye Reactive Orange 13 by newly isolated bacterial strain Alcaligenes faecalis PMS-1. Int Biodeterior Biodegrad 2012;69:41-50. https://doi.org/10.1016/j.ibiod.2012.01.002

20. Mohanty SS, Kumar A. Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes. Sci Rep 2021;11:1-15. https://doi.org/10.1038/s41598-021-87227-6

21. Zong M, Song D, Zhang X, Huang X, Lu X, Rosso KM. Facet-dependent photodegradation of methylene blue by hematite nanoplates in visible light. Environ Sci Technol 2020;55:677-88. https://doi.org/10.1021/acs.est.0c05592

22. Brito CN, Ferreira MB, de Moura Santos EC, Léon JJ, Ganiyu SO, Martínez-Huitle CA. Electrochemical degradation of Azo-dye Acid Violet 7 using BDD anode: effect of flow reactor configuration on cell hydrodynamics and dye removal efficiency. J Appl Electrochem 2018;48:1321-30. https://doi.org/10.1007/s10800-018-1257-4

23. Holt JG, Krieg NR, Sneath PH, Staley JT, Williams ST. Bergey's manual of determinative bacteriology. 9th edition, Williams &Wilkins, Baltimore, MD, p 566, 1994.

24. Das D, Banerjee D, Mondal M, Shett A, Das B, Das NS, et al. Nickel doped graphitic carbon nitride nanosheets and its application for dye degradation by chemical catalysis. Mater Res Bull 2018;101:291-304. https://doi.org/10.1016/j.materresbull.2018.02.004

25. Ganesan P, Reegan AD, David RH, Gandhi MR, Paulraj MG, Al-Dhabi NA, et al. Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India. Alexandria J Med 2017;53:101-10. https://doi.org/10.1016/j.ajme.2016.03.004

26. Zuker M. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 2003;31:3406-15. https://doi.org/10.1093/nar/gkg595

27. Karim ME, Dhar K, Hossain MT. Decolorization of textile reactive dyes by bacterial monoculture and consortium screened from textile dyeing effluent. J Genet Eng Biotechnol 2018;16:375-80. https://doi.org/10.1016/j.jgeb.2018.02.005

28. Balapure KH, Jain K, Chattaraj S, Bhatt NS, Madamwar D. Co-metabolic degradation of diazo dye-reactive blue 160 by enriched mixed cultures BDN. J Hazard Mater 2014;279:85-95. https://doi.org/10.1016/j.jhazmat.2014.06.057

29. Das S, Bandi V, Arora HS, Veligatla M, Garrison S, D'Souza F, et al. Synergistic catalytic effect of iron metallic glass particles in direct blue dye degradation. J Hazard Mater 2015;30:1121-7. https://doi.org/10.1557/jmr.2015.90

30. Mahmood R, Sharif F, Ali S, Hayyat MU. Enhancing the decolorizing and degradation ability of bacterial consortium isolated from textile effluent affected area and its application on seed germination. Sci World J 2015;2015:1-9. https://doi.org/10.1155/2015/628195

31. Ajaz M, Elahi A, Rehman A. Degradation of azo dye by bacterium, Alishewanella sp. CBL-2 isolated from industrial effluent and its potential use in decontamination of wastewater. J Water Reuse Desalin 2018;8:507-15. https://doi.org/10.2166/wrd.2018.065

32. Meerbergen K, Willems KA, Dewil R, Van Impe J, Appels L, Lievens B. Isolation and screening of bacterial isolates from wastewater treatment plants to decolorize azo dyes. J Biosci Bioeng 2018;125:448-56. https://doi.org/10.1016/j.jbiosc.2017.11.008

33. Ahmed I, Haque F, Rahman MA, Parvez MA, Mou TJ. Screening of methyl red degrading bacteria isolated from textile effluents of Savar Area, Dhaka, Bangladesh. Adv Biosci Biotechnol 2020;11:301-18. https://doi.org/10.4236/abb.2020.117022

34. Tiar A, Askarne L, Ait addi E, Assabbane A, and Boubaker H. Decolorization of the azo dye methyl red by an isolated bacterium Enterobacter hormaechei strain CUIZ1. J Mater Environ Sci 2018;9:2822-30.