Research Article | Volume 10, Supplement 2, July, 2022

Bioremediation of textile dyeing industry effluent from small-scale industries using a microbial consortium of Bacillus sp., Escherichia coli, and Aspergillus niger

Shobina Kannan Jegathambal Palanichamy T. Sugitha C. Mayilsami   

Open Access   

Published:  Jun 20, 2022

DOI: 10.7324/JABB.2022.10s211

Dyes are the visible contaminants that are released from the textile industries. Decontamination of textile dye effluents using microbes is environmentally viable over chemical, physical and other mechanical methods. Bacteria, fungi, yeast, and algae have synergistic metabolic activities that alter the chromogen and degrade the absorbed dye color. This work was aimed at investigating the dye decolorization potential of a mixed microbial culture (MMC) obtained from different soil and sludge samples. A single dye (Direct Blue 53) was used for comparison studies. The MMC were incubated for 9 days in mineral salt medium with dye and the absorbance of its filtrate at 647 nm (Blue dye) and 308 nm (Industrial dye) was noted down for every 22 h. The color removal efficiency (CRE) by MMC were 47.04%, 46.77%, 45.21%, and 35.02% for soil of textile dyeing unit (DS), sludge from STP (SE) soil (SS) from drying bed of STP and sludge from membrane reactor of dyeing unit (DE) respectively. Further, the maximum CRE of 98.35% was recorded by microbial culture from drying bed soil of STP (SS), followed by microbial culture from STP sludge (SE) was 97.96%, textile dyeing unit soil (DS) of 96.99%, and sludge form membrane reactor of dyeing unit (DE) was 96%. Bacillus sp. isolated from eco-bio block was tested against the blue dye and gave color removal of about 89.25%. The study concluded that the microbe present in soil obtained from the dyeing unit is naturally acclimatized to the dye waste and hence shows highest dye removal efficiency.

Keyword:     Biodegradation Bio sorption Decolorization Mixed microbial culture Textile dyes


Kannan S, Palanichamy J, Sugitha T, Mayilsami C. Bioremediation of textile dyeing industry effluent from small scale industries using a microbial
consortium of Bacillus sp., Escherichia coli, and Aspergillus niger. J App Biol Biotech. 2022;10(Suppl 2):100-106. DOI: 10.7324/JABB.2022.10s211

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Daniel D, Jegathambal P, Bevers B. In situ bioremediation of textile dye effluent-contaminated soils using mixed microbial culture. Int J Civ Eng 2019;17:1527-36.

2. Chequer FD, de Oliveira GR, Ferraz EA, Cardoso JC, Zanoni MB, de Oliveira DP. Textile dyes: Dyeing process and environmental impact. In: Guney M, editor. Eco-friendly Textile Dyeing and Finishing. London: Intech Open; 2013. p. 151-76.

3. Bafana A, Devi SS, Chakrabarti T. Azo dyes: Past, present and the future. Environ Rev 2011;19:350-71.

4. Sarker R, Chowdhury M, Deb AK. Reduction of color intensity from textile dye wastewater using microorganisms: A review. Int J Curr Microbiol Appl Sci 2019;8:3407-15.

5. Ali H. Biodegradation of synthetic dyes-a review. Water Air Soil Pollut 2010;213:251-73.

6. Parmar ND, Shukla SR. Biodegradation of anthraquinone based dye using an isolated strain Staphylococcus hominis subsp. hominis DSM 20328. Environ Prog Sustain Energy 2018;37:203-14.

7. Yaseen D, Scholz M. Textile dye wastewater characteristics and constituents of synthetic effluents: A critical review. Int J Environ Sci Technol 2019;16:1193-226.

8. Sarkar S, Banerjee A, Halder U, Biswas R, Bandopadhyay R. Degradation of synthetic azo dyes of textile industry: A sustainable approach using microbial enzymes. Water Conserv Sci Eng 2017;2:121-31.

9. Tang S, Xia D, Yao Y, Chen T, Sun J, Yin Y, et al. Dye adsorption by self-recoverable, adjustable amphiphilic graphene aerogel. J Colloid Interface Sci 2019;554:682-91.

10. Saxena A, Gupta S. Bioefficacies of microbes for mitigation of Azo dyes in textile industry effluent: A review. Bioresources 2020;15:9858.

11. Pandey A, Singh P, Iyengar L. Bacterial decolorization and degradation of azo dyes. Int Biodeterior Biodegradation 2007;59:73-84.

12. Blümel S, Stolz A. Cloning and characterization of the gene coding for the aerobic azoreductase from Pigmentiphaga kullae K24. Appl Microbiol Biotechnol 2003;62:186-90.

13. Chen K, Leona M, Vo?Dinh KC, Yan F, Wabuyele MB, Vo?Dinh T. Application of surface?enhanced Raman scattering (SERS) for the identification of anthraquinone dyes used in works of art. J Raman Spectrosc 2006;37:520-7.

14. Misal SA, Gawai KR. Azoreductase: A key player of xenobiotic metabolism. Bioresour Bioprocess 2018;5:1-9.

15. Pearce C, Lloyd J, Guthrie J. The removal of colour from textile wastewater using whole bacterial cells: A review. Dyes Pigm 2003;58:179-96.

16. Aamr A, Cuiling J. Bacterial influence on textile wastewater decolorization. J Environ Prot 2012;3:889-903.

17. Rajeswari K, Subashkumar R, Vijayaraman K. Biodegradation of mixed textile dyes by bacterial strains isolated from dyewaste effluent. Res J Environ Toxicol 2011;5:97-107.

18. Philip L, Murty B. Development of Mathematical Models for Clean up of Cr(vi) Contaminated Aquifers Using Bioremediation, Final Report, IIT Chennai; 2010.

19. Annuar M, Adnan S, Vikineswary S, Chisti Y. Kinetics and energetics of azo dye decolorization by Pycnoporus sanguineus. Water Air Soil Pollut 2009;202:179-88.

20. van der Zee FP, Villaverde S. Combined anaerobic-aerobic treatment of azo dyes-a short review of bioreactor studies. Water Res 2005;39:1425-40.

21. Forgacs E, Cserhati T, Oros G. Removal of synthetic dyes from wastewaters: A review. Environ Int 2004;30:953-71.

22. Chaurasia P, Bharati S, Kumar S. Microbes as remediating agents in detoxification of dyes. J Appl Biotechnol Bioeng 2020;7:242-4.

23. Verma RK, Sankhla MS, Rathod NV, Sonone SS, Parihar K, Singh GK. Eradication of fatal textile industrial dyes by wastewater treatment. Biointerface Res Appl Chem 2021;12:567-87.

24. Furuya EY, Lowy FD. Antimicrobial-resistant bacteria in the community setting. Nat Rev Microbiol 2006;4:36-45.

25. Mohanty SS, Kumar A. Enhanced degradation of anthraquinone dyes by microbial monoculture and developed consortium through the production of specific enzymes. Sci Rep 2021;11:1-15.

26. Moawad H, El-Rahim WM, Khalafallah M. Evaluation of biotoxicity of textile dyes using two bioassays. J Basic Microbiol 2003;43:218-29.

27. Zin KM, Effendi Halmi MI, Abd Gani SS, Zaidan UH, Samsuri AW, Abd Shukor MY. Microbial decolorization of triazo dye, direct blue 71: An optimization approach using response surface methodology (RSM) and artificial neural network (ANN). Biomed Res Int 2020;2020:2734135.

28. Dawkar V, Jadhav U, Jadhav S, Govindwar S. Biodegradation of disperse textile dye Brown 3REL by newly isolated Bacillus sp. VUS. J Appl Microbiol 2008;105:14-24.

29. Dhanve R, Shedbalkar U, Jadhav JP. Biodegradation of diazo reactive dye Navy Blue HE2R (Reactive Blue 172) by an isolated Exiguobacterium sp. RD3. Biotechnol Bioprocess Eng 2008;13:53-60.

30. Guibal E, Roulph C, Le Cloirec P. Infrared spectroscopic study of uranyl biosorption by fungal biomass and materials of biological origin. Environ Sci Technol 1995;29:2496-503.

31. Li W, Mu B, Yang Y. Feasibility of industrial-scale treatment of dye wastewater via bio-adsorption technology. Bioresour Technol 2019;277:157-70.

32. Junnarkar N, Murty DS, Bhatt NS, Madamwar D. Decolorization of diazo dye direct red 81 by a novel bacterial consortium. World J Microbiol Biotechnol 2006;22:163-8.

33. Kurade MB, Waghmode TR, Khandare RV, Jeon BH, Govindwar SP. Biodegradation and detoxification of textile dye disperse red 54 by brevibacillus laterosporus and determination of its metabolic fate. J Biosci Bioeng 2016;121:442-9.

34. Asses N, Ayed L, Hkiri N, Hamdi M. Congo red decolorization and detoxification by Aspergillus niger: Removal mechanisms and dye degradation pathway. Biomed Res Int 2018;2018;3049686.

Article Metrics
53 Views 53 Downloads 106 Total



Related Search

By author names