Research Article | Volume: 5, Issue: 2, March-April, 2017

Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants

N. Brijesha H. S. Aparna   

Open Access   

Published:  Mar 20, 2017

DOI: 10.7324/JABB.2017.50214
Abstract

Colostrum is the first liquid food for the new born baby, secreted after the parturition. After few days of maturation the colostrum turns to milk. The cream or fat fraction of milk consists of fat droplets composed primarily of triacylglycerols that are secreted from the apical surface of the mammary cells surrounded by cellular thin membranes membrane, the fat globule membrane (FGM), formed by proteins which have been suggested to be cholesterolemia-lowering factors, inhibitors of cancer cell growth, vitamin binders, antioxidant, bactericidal, suppressors of multiple sclerosis. FGM was isolated from buffalo colostrum and milk, the fat content was > 2-fold higher in the former than in the latter. The colostrum fat globule membrane (CFGM) fraction also showed significantly higher protein content than milk fat globule membrane (MFGM) extract. Further, the SDS-PAGE separation of the total proteins from the CFGM and MFGM showed differential banding pattern. Antioxidative property of the two FGM extracts was deliberated based on their free radical scavenging ability and reducing power. CFGM was found to exhibit a relatively higher antioxidant and reductive capacity than the MFGM fractions. The results obtained in the present study showcases the superior beneficial health effects of the CFGM over the MFGM.


Keyword:     AntioxidantsBubalus bubiliscolostrumfat globule membranemilkreducing power.


Citation:

Brijesha N, Aparna HS. Buffalo (Bubalus bubilis) colostrum and milk fat globule membrane fractions are potent antioxidants. J App Biol Biotech. 2017; 5 (02): 089-093. DOI: 10.7324/JABB.2017.50214

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text
Reference

1. FAOSTAT. Production quantity of livestock primary 2012. Food and Agriculture Organisation of the United Nations - Statistics Division (Retrieved 23/10/2014 from http://faostat3.fao.org/download/Q/QL/E). 2012.

2. Ménard O, Ahmad S, Rousseau F, Briard-bion V, Gaucheron F, Lopez,C. Buffalo vs cow milk fat globules: Size distribution , zeta-potential , compositions in total fatty acids and in polar lipids from the milk fat globule membrane. Food Chemistry. 2010; 120(2):544-551.

3. Solah VA, Staines V, Honda S, Limley H. A Measurement of Milk Color and Composition: Effect of Dietary Intervention on Western Australian Holstein‐Friesian Cow’s Milk Quality. Journal of Food Science. 2007; 72(8):S560-S566.

4. Varricchio ML, Di-Francia A, Masucci F, Romano R, Proto V. Fatty acid composition of Mediterranean buffalo milk fat Fatty acid composition of Mediterranean buffalo milk fat, Italian Journal of Animal Science. 2007; 6:509-511.

5. Gopal PK, Gill HS. Oligosaccharides and glycoconjugates in bovine milk and colostrum. Brazilian Journal of Nutrition. 2000; 84:69-74.

6. Aurelia P, Cristian C, Camelia R, Vioara M, Gheorghe M. The study of the main parameters quality of buffalo milk. African Journal of Microbiology. 2009; 10:201-206.

7. Wu CC. Proteomics reveal a link between the endoplasmic reticulum and lipid secretory mechanisms in mammary epithelial cells. Electrophoresis. 2000; 21:3470-3482.

8. Lopez C, Briad-Bion V, Menard O, Russeau F, Pradel P, Besle JM. Phospholipid, sphingolipid, and fatty acid compositions of the milk fat globule membrane are modified by diet. Journal of Agricultural and Food Chemistry. 2008; 56:5226-5236.

9. Dewettinck K, Rombaut R, Thienpont N, Le T T, Messens K and Camp JV. International Dairy Journal. 2008; 18:436-457.

10. Lopez C. Milk fat globules enveloped by their biological membrane: Unique colloidal assemblies with a specific composition and structure. Current Opinion in Colloid and Interface Science. 2011; 16:391-404.

11. Singh H. The milk fat globule membrane-A biophysical system for food applications. Current Opinion in Colloid and Interface Science. 2006; 11:154-163.

12. Mather IH, Keenan TW. Origin and secretion of milk lipids. Journal of Mammary Gland Biology and Neoplasia 1998; 3:259-273.

13. Reinhardt TA, Lippolis JD. Bovine milk fat globule membrane proteome. Journal of Dairy Research. 2006; 73:406-416.

14. Fong BY, Norris CS. Quantification of milk fat globule membrane proteins using selected reaction monitoring mass spectrometry Journal of Agricultural and Food Chemistry. 2009; 57: 6021-6028.

15. Cavaletto M, Giuffrida M G and Conti A. Milk Fat Globule Membrane Components--A Proteomic Approach. Advances in Experimental Medicine and Biology. 2008; 606:129-141.

16. Riccio P. The proteins of the milk fat globule membrane in the balance. Trends in Food Science and Technology. 2004; 15(9): 458-461.

17. Affolter M, Grass L, Vanrobaeys F, Casado B, Kussmann M. J. Qualitative and quantitative profiling of the bovine milk fat globule membrane proteome. Proteomics. 2010; 73:1079-1088.

18. Hettinga K, Van VH, De VS, Boeren S, Van HT, Van AJ and Vervoort J. The host defense proteome of human and bovine milk. PLoS One. 2011; 6, 1710-1712.

19. Kvistgaard AS, Pallesen LT, Arias CF, López S, Petersen TE, Heegaard CW and Rasmussen JT. Inhibitory effects of human and bovine milk constituents on rotavirus infections. Journal of Dairy Science. 2004; 8:4088-4096.

20. Spitsberg, VL. Bovine milk fat globule membrane as a potential nutraceutical. Journal of Dairy Science. 2005; 88(7):2289-2294.

21. Mather IH. A review and proposed nomenclature for major proteins of the milk-fat globule membrane. Journal of Dairy Science. 2000; 83:203-247.

22. Lindmark-Mansson H, Akesson B. Antioxidative factors in milk. British Journal of Nutrition. 2000; 84:S103-S110.

23. Dalbeth N, Gracey E, Pool B, Callon K, McQueen FM, Cornish J, MacGibbon A, Palmano K. Identification of dairy fractions with anti-inflammatory properties in models of acute gout. Annals of the Rheumatic Diseases. 2010; 69:766-769.

24. Park E J, Thomson AB, Clandinin R MT. Protection of intestinal occludin tight junction protein by dietary gangliosides in lipopolysaccharide-induced acute inflammation. Journal of Pediatric Gastroenterology and Nutrition. 2010; 50:321-328.

25. Alwani ME, Wu BX, Obeid LM, Hannun YA. Bioactive sphingolipids in the modulation of the inflammatory response. Pharmacology and Therapeutics. 2006; 112:171-183.

26. Fortunato D, Giuffrida M. G, Cavaletto M, Garoffo L. P, Dellavalle G, Napolitano L, Giunta C, Fabris C, Bertino E, Coscia A. Structural proteome of human colostral fat globule. Proteomics . 2003; 897-905.

27. Reinhardt T, Lippolis J. D. Developmental changes in the milk fat globule membrane proteome during the transition from colostrum to milk. Journal of Dairy Science. 2008; 91 (6), 2307-2318.

28. Yang M, Cong M, Peng X, Wu J, Wu R, Liu B, Yue X. Quantitative proteomic analysis of milk fat globule membrane (MFGM) proteins in human and bovine colostrum and mature milk samples through iTRAQ labeling. Food and function. 2016; 2438-2450.

29. Basch JJ, Greenberg R, Farrell HM. Identification of the milk fat globule membrane proteins.: II. Isolation of major proteins from electrophoretic gels and comparison of their amino acid compositions. Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology, 1985; 830(2): 127-135.

30. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 1951; 193:266-275.

31. Weber K, Osborn M. The reliability of molecular weight determinations by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry. 1969; 244: 4406-4412.

32. Pulido R, Bravo L, Saura CF. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry. 2000; (48):396-3402.

33. Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilizing activity - a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil, Fitoterapia. 1999; 70:251-257.

34. Pawar N, Kogje A, Bhondave P, Nagarkar B, Kulkarni O, Harsulkar A, Jagtap S. Comparative free radical scavenging and anti-inflammatory potential of branded market samples of an ayurvedic formulations: Dashamoolarishta. International Journal of Pharma and Bio Sciences. 2013; 4(1):789-799.

35. Jensen RG, Ferris AM, Lammi-Keefe CJ. The composition of milk fat. Journal of Dairy Science. 1991; 74:3228−3243.

36. Chen J, Lindmark-Mansson H, Gorton L, Åkesson B. Antioxidant capacity of bovine milk as assayed by spectrophotometric and amperometric methods. International Dairy Journal. 2003; 13:927−935.

Article Metrics
125 Views 94 Downloads 219 Total

Year

Month

Related Search

By author names

Similar Articles