Sensing oxy anodic microbial fuel cell potential of halotolerant protease producer Priestia megaterium BorS17B13 belonging to mangrove biota

Priyanka S. Sawant; Jignasha T. Thumar

doi:10.7324/JABB.2023.152337

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Abstract

Exploring the uncharted nooks of mangrove-rich biota is the key to unlocking the hidden world of industrial opportunities and keeping up with demanding products like microbial-based enzymes. A salt-tolerant bacteria, Priestia megaterium strain, BorS17B13 was isolated from the mangrove-rich biota of Borivali monari creek, Maharashtra, India. The organism was Gram-positive, aerobic, and capable of secreting protease enzymes in abundance using a crude source (wheat flour) as the sole source of carbon and nitrogen. Optimum protease activity was 739 U/mL at 72 h. Further, its potential was securitized for bioenergy production in a dual chamber of the oxy anodic microbial fuel cell (OA-MFC) with an impeller for analog configurations, like a bioreactor, to achieve homogenized medium. The maximum OCV was 614 mV and the SCC was 3.5 Am-3; they were also studied with an external load/resistor (47 ohm and 100 ohm). The maximum power output was 454 mW, and the volumetric power density was 0.65 mW/m3 (100 ohm). The novel concept of OA-MFCs will highlight the potential for combining technologies and will take aerobic microbes strands into the future by utilizing MFC techniques into “Aerobic biosensors.” Our research as a whole is attractive, as mangrove-associated halotolerant microbes are rarely explored for their OA-MFC potential to date.

Keyword: Protease Halotolerant Extremophile Oxy-anode microbial fuel cell Crude sources Wheat flour Microbial fuel cell

Citation:

Sawant PS, Thumar J. Sensing oxy anodic microbial fuel cell potential of halotolerant protease producer Priestia megaterium BorS17B13 belonging to mangrove biota. J App Biol Biotech. 2023;11(6):249-254. https://doi.org/10.7324/JABB.2023.152337

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Reference

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2. Nagelkerken I, Blaber SJ, Bouillon S, Green P, Haywood M, Kirton LG, et al. The habitat function of mangroves for terrestrial and marine fauna: A review. Aquat Bot 2008;89:155-85. https://doi.org/10.1016/j.aquabot.2007.12.007
3. Gupta R, Beg QK, Lorenz P. Bacterial alkaline proteases: Molecular approaches and industrial applications. Appl Microbiol Biotechnol 2002;59:15-32. https://doi.org/10.1007/s00253-002-0975-y
4. Roller SD, Bennetto HP, Delaney GM, Mason JR, Stirling JL, Thurston CF. Electron-transfer coupling in microbial fuel cells: 1. Comparison of redox-mediator reduction rates and respiratory rates of bacteria. J Chem Technol Biotechnol Biotechnol 1984;34:3-12. https://doi.org/10.1002/jctb.280340103
5. Liu H, Logan BE. Electricity generation using an air-cathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane. Environ Sci Technol 2004;38:4040-6. https://doi.org/10.1021/es0499344
6. Liu H, Ramnarayanan R, Logan BE. Production of electricity during wastewater treatment using a single chamber microbial fuel cell. Environ Sci Technol 2004;38:2281-5. https://doi.org/10.1021/es034923g
7. Rabaey K, Lissens G, Siciliano SD, Verstraete W. A microbial fuel cell capable of converting glucose to electricity at high rate and efficiency. Biotechnol Lett 2003;25:1531-5. https://doi.org/10.1023/A:1025484009367
8. Thumar JT, Dhulia K, Singh SP. Isolation and partial purification of an antimicrobial agent from halotolerant alkaliphilic Streptomyces aburaviensis strain Kut-8. World J Microbiol Biotechnol 2010;26:2081-7. https://doi.org/10.1007/s11274-010-0394-7
9. Babu GS, Kiran RR, Lokeswari N, Karothi JR. Optimization of protease production from Aspergillus Oryzae sp. using box-behnken experimental design. J Chem 2007;4:145-53. https://doi.org/10.1155/2007/254613
10. Thumar JT, Sawant PS. Amalgamation of technologies and scientrepreneur prospective of electrochemically active halophilic microbugs-a review paper. Vidya A J Gujarat Univ 2023;17:125-56.
11. Thumar JT, Singh SP. Secretion of an alkaline protease from a salttolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1. Braz J Microbiol 2007;38:766-72. https://doi.org/10.1590/S1517-83822007000400033
12. Patel RK, Dodia MS, Joshi RH, Singh SP. Production of extracellular halo-alkaline protease from a newly isolated haloalkaliphilic Bacillus sp. isolated from seawater in Western India. World J Microbiol Biotechnol 2006;22:375-82. https://doi.org/10.1007/s11274-005-9044-x
13. Hagihara B. In: Boyer PD, editor. The Enzymes. 2nd ed., Vol. 4. New York: Academic Press; 1958.
14. Joo HS, Chang CS. Production of protease from a new alkalophilic Bacillus sp. I-312 grown on soybean meal: Optimization and some properties. Process Biochem 2005;40:1263-70. https://doi.org/10.1016/j.procbio.2004.05.010
15. Chu WH. Optimization of extracellular alkaline protease production from species of Bacillus. J Ind Microbiol Biotechnol 2007;34:241-5. https://doi.org/10.1007/s10295-006-0192-2
16. Mehta VJ, Thumar JT, Singh SP. Production of alkaline protease from an alkaliphilic actinomycete. Bioresour Technol 2006;97:1650-4. https://doi.org/10.1016/j.biortech.2005.07.023
17. Vijay A, Arora S, Gupta S, Chhabra M. Halophilic starch degrading bacteria isolated from Sambhar Lake, India, as potential anode catalyst in microbial fuel cell: A promising process for saline water treatment. Bioresour Technol 2017;256:391-8. https://doi.org/10.1016/j.biortech.2018.02.044
18. Gajda I, Obata O, Greenman J, Ieropoulos IA. Electroosmotically generated disinfectant from urine as a by-product of electricity in microbial fuel cell for the inactivation of pathogenic species. Sci Rep 2020;10:5533. https://doi.org/10.1038/s41598-020-60626-x
19. Koók L, Nemestóthy N, Bélafi-Bakó K, Bakonyi P. The influential role of external electrical load in microbial fuel cells and related improvement strategies: A review. Bioelectrochemistry 2021;140:107749. https://doi.org/10.1016/j.bioelechem.2021.107749
20. Klevinskas A, Kantminien? K, Žmuidzinavi?ien? N, Jonuškien? I, Griškonis E. Microbial fuel cell as a bioelectrochemical sensor of nitrite ions. Processes 2021;9:1330. https://doi.org/10.3390/pr9081330
21. Koffi NJ, Okabe S. High voltage generation from wastewater by microbial fuel cells equipped with a newly designed low voltage booster multiplier (LVBM). Sci Rep 2020;10:18985. https://doi.org/10.1038/s41598-020-75916-7
22. Lyon DY, Buret F, Vogel TM, Monier JM. Is resistance futile Changing external resistance does not improve microbial fuel cell performance. Bioelectrochemistry 2010;78:2-7. https://doi.org/10.1016/j.bioelechem.2009.09.001
23. Katuri KP, Scott K, Head IM, Picioreanu C, Curtis TP. Microbial fuel cells meet with external resistance. Bioresour Technol 2011;102:2758-66. https://doi.org/10.1016/j.biortech.2010.10.147
24. Stein NE, Hamelers HV, Buisman CN. The effect of different control mechanisms on the sensitivity and recovery time of a microbial fuel cell based biosensor. Sens Actuators B Chem 2012;171-2:816-21. https://doi.org/10.1016/j.snb.2012.05.076
25. Freguia S, Rabaey K, Yuan Z, Keller J. Non-catalyzed cathodic oxygen reduction at graphite granules in microbial fuel cells. Electrochim Acta 2007;53:598-603. https://doi.org/10.1016/j.electacta.2007.07.037
26. Simeon MI, Asoiro FU, Aliyu M, Raji OA, Freitag R. Polarization and power density trends of a soil-based microbial fuel cell treated with human urine. Int J Energy Res 2020;44:5968-76. https://doi.org/10.1002/er.5391

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1. Alongi DM. Mangrove forests: Resilience, protection from tsunamis, and responses to global climate change. Estuar Coast Shelf Sci 2008;76:1-13. https://doi.org/10.1016/j.ecss.2007.08.024

2. Nagelkerken I, Blaber SJ, Bouillon S, Green P, Haywood M, Kirton LG, et al. The habitat function of mangroves for terrestrial and marine fauna: A review. Aquat Bot 2008;89:155-85. https://doi.org/10.1016/j.aquabot.2007.12.007

3. Gupta R, Beg QK, Lorenz P. Bacterial alkaline proteases: Molecular approaches and industrial applications. Appl Microbiol Biotechnol 2002;59:15-32. https://doi.org/10.1007/s00253-002-0975-y

4. Roller SD, Bennetto HP, Delaney GM, Mason JR, Stirling JL, Thurston CF. Electron-transfer coupling in microbial fuel cells: 1. Comparison of redox-mediator reduction rates and respiratory rates of bacteria. J Chem Technol Biotechnol Biotechnol 1984;34:3-12. https://doi.org/10.1002/jctb.280340103

5. Liu H, Logan BE. Electricity generation using an air-cathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane. Environ Sci Technol 2004;38:4040-6. https://doi.org/10.1021/es0499344

6. Liu H, Ramnarayanan R, Logan BE. Production of electricity during wastewater treatment using a single chamber microbial fuel cell. Environ Sci Technol 2004;38:2281-5. https://doi.org/10.1021/es034923g

7. Rabaey K, Lissens G, Siciliano SD, Verstraete W. A microbial fuel cell capable of converting glucose to electricity at high rate and efficiency. Biotechnol Lett 2003;25:1531-5. https://doi.org/10.1023/A:1025484009367

8. Thumar JT, Dhulia K, Singh SP. Isolation and partial purification of an antimicrobial agent from halotolerant alkaliphilic Streptomyces aburaviensis strain Kut-8. World J Microbiol Biotechnol 2010;26:2081-7. https://doi.org/10.1007/s11274-010-0394-7

9. Babu GS, Kiran RR, Lokeswari N, Karothi JR. Optimization of protease production from Aspergillus Oryzae sp. using box-behnken experimental design. J Chem 2007;4:145-53. https://doi.org/10.1155/2007/254613

10. Thumar JT, Sawant PS. Amalgamation of technologies and scientrepreneur prospective of electrochemically active halophilic microbugs-a review paper. Vidya A J Gujarat Univ 2023;17:125-56.

11. Thumar JT, Singh SP. Secretion of an alkaline protease from a salttolerant and alkaliphilic, Streptomyces clavuligerus strain Mit-1. Braz J Microbiol 2007;38:766-72. https://doi.org/10.1590/S1517-83822007000400033

12. Patel RK, Dodia MS, Joshi RH, Singh SP. Production of extracellular halo-alkaline protease from a newly isolated haloalkaliphilic Bacillus sp. isolated from seawater in Western India. World J Microbiol Biotechnol 2006;22:375-82. https://doi.org/10.1007/s11274-005-9044-x

13. Hagihara B. In: Boyer PD, editor. The Enzymes. 2nd ed., Vol. 4. New York: Academic Press; 1958.

14. Joo HS, Chang CS. Production of protease from a new alkalophilic Bacillus sp. I-312 grown on soybean meal: Optimization and some properties. Process Biochem 2005;40:1263-70. https://doi.org/10.1016/j.procbio.2004.05.010

15. Chu WH. Optimization of extracellular alkaline protease production from species of Bacillus. J Ind Microbiol Biotechnol 2007;34:241-5. https://doi.org/10.1007/s10295-006-0192-2

16. Mehta VJ, Thumar JT, Singh SP. Production of alkaline protease from an alkaliphilic actinomycete. Bioresour Technol 2006;97:1650-4. https://doi.org/10.1016/j.biortech.2005.07.023

17. Vijay A, Arora S, Gupta S, Chhabra M. Halophilic starch degrading bacteria isolated from Sambhar Lake, India, as potential anode catalyst in microbial fuel cell: A promising process for saline water treatment. Bioresour Technol 2017;256:391-8. https://doi.org/10.1016/j.biortech.2018.02.044

18. Gajda I, Obata O, Greenman J, Ieropoulos IA. Electroosmotically generated disinfectant from urine as a by-product of electricity in microbial fuel cell for the inactivation of pathogenic species. Sci Rep 2020;10:5533. https://doi.org/10.1038/s41598-020-60626-x

19. Koók L, Nemestóthy N, Bélafi-Bakó K, Bakonyi P. The influential role of external electrical load in microbial fuel cells and related improvement strategies: A review. Bioelectrochemistry 2021;140:107749. https://doi.org/10.1016/j.bioelechem.2021.107749

20. Klevinskas A, Kantminien? K, Žmuidzinavi?ien? N, Jonuškien? I, Griškonis E. Microbial fuel cell as a bioelectrochemical sensor of nitrite ions. Processes 2021;9:1330. https://doi.org/10.3390/pr9081330

21. Koffi NJ, Okabe S. High voltage generation from wastewater by microbial fuel cells equipped with a newly designed low voltage booster multiplier (LVBM). Sci Rep 2020;10:18985. https://doi.org/10.1038/s41598-020-75916-7

22. Lyon DY, Buret F, Vogel TM, Monier JM. Is resistance futile Changing external resistance does not improve microbial fuel cell performance. Bioelectrochemistry 2010;78:2-7. https://doi.org/10.1016/j.bioelechem.2009.09.001

23. Katuri KP, Scott K, Head IM, Picioreanu C, Curtis TP. Microbial fuel cells meet with external resistance. Bioresour Technol 2011;102:2758-66. https://doi.org/10.1016/j.biortech.2010.10.147

24. Stein NE, Hamelers HV, Buisman CN. The effect of different control mechanisms on the sensitivity and recovery time of a microbial fuel cell based biosensor. Sens Actuators B Chem 2012;171-2:816-21. https://doi.org/10.1016/j.snb.2012.05.076

25. Freguia S, Rabaey K, Yuan Z, Keller J. Non-catalyzed cathodic oxygen reduction at graphite granules in microbial fuel cells. Electrochim Acta 2007;53:598-603. https://doi.org/10.1016/j.electacta.2007.07.037

26. Simeon MI, Asoiro FU, Aliyu M, Raji OA, Freitag R. Polarization and power density trends of a soil-based microbial fuel cell treated with human urine. Int J Energy Res 2020;44:5968-76. https://doi.org/10.1002/er.5391