Research Article | Volume: 3, Issue: 1, Jan-Feb, 2015
In vitro plant regeneration in Capsicum chinense Jacq. (Naga Chili)
An in vitro plantlet regeneration protocol was developed for Capsicum chinense Jacq. Naga Chili, one of the world’s hottest chili cultivars and an important horticultural crop of Northeast India. In vitro propagation plays a major role in conservation of genetically pure plants, crop improvement and production of disease free planting materials. The effect of different compositions of plant growth regulators on multiple shoot development and callus induction was investigated. Multiple shoot was induced by culturing explants in MS medium supplemented with Benzyl adenine (BA) in combination with Indole-3-acetic acid (IAA). Maximum numbers of shoot buds were induced in MS medium containing 5 mgl-1 BA and 0.5 mgl-1 IAA. Successful induction of callus from stem segments of in vitro raised plants were achieved in MS medium in combination with 3 mgl-1Benzyl adenine (BA) and 1 mgl-1 1-Naphthaleneacetic acid (NAA).Shoot elongation and rooting were achieved in MS basal medium. This is the first successful report of plant regeneration from calluses in Capsicum chinense Jacq. Naga Chili. This protocol can be used as a cost effective method for the production of disease free planting materials and for genetic improvement and conservation of the crop.
Rahul P. Raj, Glint V. D., K. Nirmal Babu. Plant regeneration in Capsicum chinense Jacq. cv. Naga King Chili in vitro. J App Biol Biotech, 2015; 3 (01): 030-033. DOI: 10.7324/JABB.2015.3106
1. Tandon P, Kumaria S. Prospects of Plant Conservation Biotechnology in India with special reference to Northeastern Region. In: Tandon P, Sharma M, Swarup R (eds) Biodiversity: status and prospects. Narosa Publishing House, New Delhi. 2005, 79–92
2. Bhagowati, R. R., &Changkija, S. Genetic variability and traditional practices in Naga King Chili landraces of Nagaland. Asian Agri-History. 2009: 13(3):171-180
3. Morrison, R.A., Koning, R.E., and Evans, D.A. Pepper. In: Evans, D.A., Sharp, W.R., and Ammirato, P.V. (eds). Handbook of Plant Cell Culture. vol 4. McGraw Hill, New York. 1986: 554-573.
4. Agrawal, S., Chandra, N., & Kothari, S. L. Plant regeneration in tissue cultures of pepper (Capsicum annuum L. Mathania). Plant cell, tissue and organ culture. 1989: 16 (1): 47-55.
5. Ramírez-Malagón, R., & Ochoa-Alejo, N. An improved and reliable chili pepper (Capsicum annuum L.) plant regeneration method. Plant cell reports. 1996:16(3-4): 226-231.
6. Venkataiah, P., &Subhash, K. Research Articles: Thidiazuron Induced High Frequency Adventitious Shoot Formation and Plant Regeneration in Capsicum annuum L. Journal of Plant Biotechnology. 2003:5(4): 245-250.
7. Subhash, K., & Christopher, T. Direct Plantlet Formation In Cotyledon Cultures Of Capsicum-Frutescens. Current Science. 1988: 57(2): 99-100.
8. Reddy, B. O., Giridhar, P., &Ravishankar, G. A. The effect of triacontanol on micropropagation of Capsicum frutescens and Decalepis hamiltonii W & A. Plant cell, tissue and organ culture. 2002: 71(3): 253-258.
9. Sanatombi, K., & Sharma, G. J. In vitro propagation of Capsicum chinense Jacq. Biologia Plantarum. 2008;52(3): 517-520.
10. Santana-Buzzy, N., Canto-Flick, A., Barahona-Pérez, F., del Carmen Montalvo-Peniche, M., Zapata-Castillo, P. Y., Solís-Ruiz, A., & de Lourdes Miranda-Ham, M. Regeneration of habanero pepper (Capsicum chinense Jacq.) via organogenesis. HortScience, 2005; 40(6): 1829-1831.
11. Kehie, M., Kumaria, S., & Tandon, P. In vitro plantlet regeneration from cotyledon segments of Capsicum chinense Jacq. Naga King Chili, and determination of capsaicin content in fruits of in vitro propagated plants by High Performance Liquid Chromatography. Scientia Horticulturae. 2013; 164: 1-8.
12. Murashige, T., & Skoog, F. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum. 1962; 15(3): 473-497.
13. Ochoa-Alejo, N., & Ramirez-Malagon, R. In vitro chili pepper biotechnology. In Vitro Cellular & Developmental Biology-Plant. 2001; 37(6): 701-729.
14. Sanatombi, K., & Sharma, G. J. In vitro regeneration and mass multiplication of Capsicum annuum L. Journal of Food Agriculture and Environment. 2006; 4(1): 205.
15. Santana-Buzzy, N., Canto-Flick, A., Barahona-Pérez, F., del Carmen Montalvo-Peniche, M., Zapata-Castillo, P. Y., Solís-Ruiz, A.,& de Lourdes Miranda-Ham, M. Regeneration of habanero pepper (Capsicum chinense Jacq.) via organogenesis. HortScience. 2005; 40(6): 1829-1831.
16. Kehie, M., Kumaria, S., &Tandon, P. In vitro plantlet regeneration from nodal segments and shoot tips of Capsicum chinense Jacq. cv. Naga King Chili. 3 Biotech. 2012; 2(1):31-35.
17. Mangang, R. J. In vitro Callus Induction of Placental Tissues of Capsicum chinense Jacq. cv. ‘Umorok’using Different Concentrations and Combinations of Growth Hormones. International Journal of Interdisciplinary and Multidisciplinary Studies. 2014; 1(8):63-66
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