Home >Archive

Volume: 2, Issue: 6, Nov-Dec, 2014

Nov-Dec, 2014

DOI: 10.7324/JABB.2014.2604

Research Article

Authentication of “Chirayat” complex by high resolution seed morphology

Bharati Rajput, Vinaya Ghate

  Author Affiliations


Twelve different species belonging to Andrographis, Enicostema, Exacum, Indoneesiella, Peristrophe and Swertia are used as crude drugs under the common name Chirayat depending on region specific availability and diverse traditions all over the country. The crude drug is available in the local market in the form of whole plant consisting of stems, twigs with flowers and fruits. Microscopic characterization of powder sample revealed the presence of seeds with beautiful surface ornamentation. The observations indicated potential for using seed morphology as identification character. The seed surface morphology of twelve species was studied using light microscope (LM) and scanning electron microscope (SEM). The studies revealed species specific variations with reference to shape, size, colour and particularly surface. Authentication keys for Chirayat complex based on variations have been proposed.


Chirayat species, seed morphology, surface structure.

Citation: Bharati Rajput, Vinaya Ghate. Authentication of “Chirayat” complex by high resolution seed morphology. J App Biol Biotech. 2014; 2 (06): 014-017. DOI: 10.7324/JABB.2014.2604

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.


1. Bahadur B., Venkateshwarlu K. and Rama Swamy N, SEM Studies of seeds in nine species of Petunia Juss (Solanaceae). Phytomorphology.1989; 39 (2, 3): 121-128.

2. Bahadur B., Bhasker K. V. and Forooqui S. M., The taxonomic significance of seed and seed coat ( LM & SEM ) characters in six species of Nigella ( Ranunculaceae). Proc. Indian Acad. Sci. 1983; B 92: 429-435.

3. Das S. , Mitra S. K. and Mukherjee K. K., SEM studies on seed coat of some species of Ipomoea (Convolvulaceae). Phytomorphology. 1995; 45 (1 & 2): 113-125.

4. Jha S. S. and Pandey A. K., Seed coat structure in Melilotus (Fabaceae). Phytomorphology. 1989; 39 (2, 3): 221-229.

5. Paul T. K. and Nayar M. P., A scanning electron microscope survey of seed surface morphology of some taxa of Malvaceae. Bull. Bot. Surv. India. 1987; 29 (1-4): 226-252.

6. Trivedi B. S., Bagchi G. D. and Bajpai Usha. Studies on seed and spermoderm structure of Bauhinia. Phytomorphology. 1980; 30: 11-16.

7. Trivedi B. S, Bagchi G. D. and Bajpai Usha. SEM studies on the spermoderm of Sesbania Scop. (Leguminosae). Curr.Sci. 1978; 47: 599-600.

8. Garg S. Gentianaceae of N.W. Himalaya (Revision), (Today and Tomarrow Publisher, New Delhi. 1987.

9. Singh N.P., Lakshminarasimhan P. and Prasanna P. V. Flora of Maharashtra State Dicotyledones Vol 2. (BSI. Calcutta.). 2001.

Article Metrics

Similar Articles