Research Article | Volume: 6, Issue: 1, Jan-Feb, 2018

Location and histopathology of seed-borne bacterial pathogen Pseudomonas syringae pv. pisi carried by pea seeds

Ashwani Kumar Verma Kailash Agrawal   

Open Access   

Published:  Jan 17, 2018

DOI: 10.7324/JABB.2018.60104
Abstract

The present study aims to determine the location and histopathology of seed-borne bacterial pathogen Pseudomonas syringae pv. pisi associated with pea seeds. The pea seeds categorized (asymptomatic, moderately discolored, and shrivelled discolored seeds) and seeds of each category were assayed for the presence of bacterial pathogen P. syringae pv. pisi. Seed samples carrying high incidence (89.5% and 91.34%) of the bacterial pathogen were used for the microtome sectioning individually. It was observed that the cells of P. syringae pv. pisi were found in large numbers in radicle and hilum region and space between seed coat and spermoderm in shrivelled discolored seed category while in moderately discolored seed category bacterial cells were less in numbers and found into the inner side of seed coat and endosperm. Bacterial colonization within seed tissue caused necrosis, formation of lytic cavities, and reduction in cell contents.


Keyword:     Categorization Incidence Pea seeds Pseudomonas syringae pv. pisi Symptoms.


Citation:

Verma AK, Agrawal K. Location and histopathology of seed-borne bacterial pathogen Pseudomonas syringae pv. pisi carried by pea seeds. J App Biol Biotech. 2018;6(1):20-22. DOI: 10.7324/JABB.2018.60104

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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