Research Article | Volume: 5, Issue: 6, Nov-Dec, 2017

Preventive and curative control of sclerotium rot disease of cocoyam cormel (Colocasia esculenta [L., Scott]) using plant extracts and Trichoderma koningii

Nwauzoma Akagbuo Bartholomew Jaja Tamunodiari Emylia Njoku Chibuzor   

Open Access   

Published:  Nov 09, 2017

DOI: 10.7324/JABB.2017.50606

Aqueous leaf extracts from air-dried samples of Carica papaya, Chromolaena odorata, and Azadirachta indica were screened for antifungal properties against Sclerotium rolfsii at 50, 70, and 100% concentrations and Trichoderma koningii (4.9 × 106 spores/ml) in vivo. Dithane M45 at 2% concentration was used as positive control for better comparison. The aqueous extracts and T. koningii were applied as preventive and curative methods by spraying fresh and healthy cormels with S. rolfsii (3.75 × 105 spores/ml). The results showed that Mancozeb and T. koningii had significant effect (preventive and curative) on disease severity and percentage weight loss. Furthermore, preventive and curative application of T. koningii significantly reduced disease severity. C. papaya at 50% concentration (preventive method) had the highest disease severity (3.8%), as to the control (4.0%). The extracts and dithane M45 were more effective as curative, while T. koningii was better as preventive method. The result on percentage weight loss showed that C. odorata (70% concentration) had the highest (49.61%), followed by C. papaya (50%) with 47.85%, and the least was A. indica (23.77%) at 70%. C. odorata and A. indica 50% and 100% concentrations, respectively, significantly reduced weight loss, while C. odorata (100% concentration) and the control gave the highest percentage weight loss in the curative method. Conclusively, plant extracts and T. koningii were more effective in controlling the disease and reduction in weight loss than mancozeb.

Keyword:     Sclerotium rolfsii Colocasia esculenta Plant extracts Curative Preventive control.


Nwauzoma AB, Jaja ET, Njoku C. Preventive and curative control of sclerotium rot disease of cocoyam cormel (Colocasia esculenta [L., Scott]) using plant extracts and Trichoderma koningii. J App Biol Biotech. 2017;5(6):40-44. DOI: 10.7324/JABB.2017.50606.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Onyeka J. Status of Cocoyam (Colocasia esculenta and Xanthosoma spp) in West and Central Africa: Production, Household Importance and the Threat from Leaf Blight. Lima (Peru): CGIAR Research Program on Roots, Tubers and Bananas (RTB); 2014. p. 32.

2. Eze CC, Maduewesi JN. Relation of traditional methods to the magnitude of storage losses of cocoyam (Colocasia esculenta (L) schott). Nig J Plant Protection 1990;13:26-34.

3. Nwachukwu EO, Osuji JO. Evaluation of plant extracts for antifungal activity against Sclerotium rolfsii causing cocoyam cormel rot in storage. Res J Agric Biol Sci 2008;4:787-93.

4. Olurinola PF, Ehinmidu JO, Bonire JJ. Antifungal activity of n-tributyltin acetate against some common yam rot fungi. Appl Environ Microbiol 1992;58:758-60.

5. Onuegbu BA. Composition of four cocoyam cultivars and their tolerance to corm rot. Trop Sci 1999;39:136-9.

6. Arunasri PT, Chalam NP, Redddy ST, Reddy BR. Investigations on fungicidal sensitivity of Trichoderma spp. and Sclerotium rolfsii (collar rot pathogen) in Crossandra. Int J Appl Biol Pharmaceut Tech 2011;2:290-3.

7. Maxwell DP. Oxalic acid production by Sclerotinia sclerotiorum in infected bean and in culture. Phytopathology 1970;60:1395-8.

8. Okigbo RN, Odurukwe CN. Occurrence and control of fungal rot pathogens of yam (Dioscorea rotundata poir) with leaf extracts of Chromolena odorata, Carica papaya and Aspilia africana. Nig J Mycol 2009;2:154-65.

9. Brocklehurst K, Salih E. Fresh non-fruit latex of Carica papaya contains papain multiple forms of chymopapain and papaya proteinase omega. Biochem J 1985;228:525-7.

10. Elisa FR, Jessica KK. Antibacterial activity of papaya leaf extracts against pathogenic bacteria. Makara J Technol 2011;15:84-5.

11. Nkuo-Akenji T, Ndip R, McThomas A, Fru EC. Anti-salmonella activity of medicinal plants from Cameroon. Cent Afr J Med 2001;47:155-8.

12. Gangulin S. “Neem” a therapeutic for all seasons. Curr Sci 2002;82:1304.

13. Moharam MH, Obiadalla-Ali HA. Preventative and curative effects of several plant derived agents against powdery mildew disease of okra. Not Sci Biol 2012;4: 76-82.

14. Phan TT, Wang L, See P, Grayer RJ, Chan SY, Lee ST. Phenolic compounds of Chromolaena odorata protect cultured skin cells from oxidative damage: Implication for cutaneous wound healing. Biol Pharm Bull 2001;24:1373-9.

15. Vital PG, Rivera WL. Antimicrobial activity and cytotoxicity of Chromolaena odorata (L.) Extracts. J Med Plant Res 2009;3:511-8.

16. Nghiem DP. The Therapeutic Effects of the Extract from the Leaves of Eupatorium odoratum on the Infection Soft Tissue and Non- Healing Wounds (PhD Thesis) 1992; Hanoi, Vietnam.

17. Wollenweber E, Dorr M, Muniappan R. Exudate flavonoids in a tropical weed, Chromolaena odorata. Biochem Syst Ecol 1996;23:873-4.

18. Olufolaji DB, Martins AA, Toyosi CA, Rowland M. Green Technologies for Sustainable Growth of Sugar and Integrated Industries in Developing Countries. China: Nanning, PR; 2014. p. 454-6.

19. Ritchie B. Practical Techniques in Plant Pathology. Wallingford U.K.: CAB; 1991.

20. Sung-Chul Y. Selection and a 3-year field trial of Sorangium cellulosum KYC 3262 against anthracnose in hot pepper. Plant Pathol J 2014;30:279-87.

21. Boungab K, Tadjeddine A, Belabid L, Fortas Z, Bassam B. Exploitation of some plant extracts for ecofriendly management of net blotch of Barley. J Chem Pharm Res 2015;7:732-9.

22. Eze SC, Ugwuoke KI, Ogbonna PE, Onah RN, Onyeonagu CC, Oyeke CC. Evaluation of indigenous technologies of fresh cocoyam (Colocasia esculenta) [(L.) (Schott)] in Southeastern Nigeria. Afr J Agric Res 2015;10:737-41.

23. Eze CS. Studies on Microbial Rotting of Cocoyam (Colocasia esculenta L.) in Storage at Nsukka. Ph.D. Thesis 1991, Nsukka, Nigeria: Department of Botany, University of Nigeria.

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