Research Article | Volume: 5, Issue: 3, May-June, 2017

In Vitro Antidiabetic and Anti-oxidant Activities of Methanol Extract of Tinospora Sinensis

Anindita Banerjee Bithin Maji Sandip Mukherjee Kausik Chaudhuri Tapan Seal   

Open Access   

Published:  Jun 19, 2017

DOI: 10.7324/JABB.2017.50311

In the present study the methanol extracts of Tinospora sinensis Lour (Merr.) was studied for alpha (α)-amylase and alpha (α)-glucosidase inhibition using an in vitro model. The plant extracts were also examined for its antioxidant activities by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method, ABTS radical scavenging ability, reducing power capacity, estimation of total phenolic content, flavonoid content and flavonol content. The study revealed that the different concentrations of the extract possessed a very good amount of total phenolics, flavonoid and flavonol and exhibit potent radical scavenging activity using DPPH and ABTS as substrate. The methanol extract exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value 0.75µg and 0.80 µg dry extract respectively and well compared with standard acarbose drug. Thus, it could be concluded that due the presence of antioxidant components the plant extract have well prospective for the management of hyperglycemia, diabetes and the related condition of oxidative stress. This knowledge will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics.

Keyword:     Tinospora sinensis Methanol extract Antioxidant activities α-amylase inhibitory activity α-glucosidase inhibitory activity.


Banerjee A, Maji B, Mukherjee S, Chaudhuri K, Seal T. In Vitro Antidiabetic and Anti-oxidant Activities of Methanol Extract of Tinospora Sinensis. J App Biol Biotech. 2017; 5 (03): 061-067.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. Keerthana G, Kalaivani MK, Sumathy A: In-vitro alpha amylase inhibitory and anti-oxidant activities of ethanolic leaf extract of Croton bonplandianum. Asian J Pharm Clin Res, 2013; 6( 4): 32-36.

2. Groop L, Forsblom C, Lehtovirta M: Characterization of the prediabetic state. Am J Hypertens, 1997; 10: 172-180.

3. Perfetti R, Barnett PS, Mathur R, Egan JM: Novel therapeutic strategies for the treatment of type 2 diabetes. Diabetes Metab Rev, 1998; 14: 207-225.

4. Alexander R: Maltodextrins: production, properties and applications.In: Schenk F, Hebeda R (ed.) Starch hydrolysis products; worldwide technology: production and applications, New York, 1992. p. 62–122.

5. Dineshkumar B, Mitra A, Manjunatha.M: A comparative study of alpha amylase inhibitory activities of common antidiabetic plants of Kharagpur 1 block. Int J Green Pharm,. 2010 ; 4: 115-21.

6. Cheng AYY, Fantus IG: Oral antihyperglycemic therapy for type 2 diabetes mellitus. Canadian Medicinal Association Journal, 2005 ; 172(2): 213–226.

7. Grover JK, Yadav S, Vats V: Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol., 2002 ; 81: 81–100.

8. Mukherjee PK, Maiti K, Mukherjee K, Houghton PJ: Leads from Indian medicinal plants with hypoglycemic potentials. J Ethnopharmacol. 2006; 106(1): 1–28.

9. Sunil K, Rashmi Kumar D: Evaluation of anti diabetic activity of Euphorbia hirta Linn. in streptozotocin induced diabetic mice. Indian Journal of Natural Products and Resources, 2010 ; 1: 200-03.

10. Rathi Sre PR, Sheila T , Kandasamy M: Phytochemical screening and "in-vitro" anti-oxidant activity of methanolic root extract of Erythrina indica. Asian Pac J Trop Biomed, 2012 ; S 1696-1700.

11. Udayan, PS.: Tinospora sinensis (Lour.) Merr. From sickupara, kollohills forest, Namakkal District Tamilnadu. Zoo’s Print Journal 2004, 19 (9): 1622- 1623.

12. Singleton V L., Rossi J A: Colorimetry of total phenolics with Phosphomolybdic-phosphotungstic acid reagents. Am.J.Enol.Vitic., 1965 ; 16: 144-158.

13. Ordonez AAL, Gomez J G, Vattuone M A, Isla M I: Antioxidant activities of Sechium edule (Jacq.) Swart extracts. Food Chem., 2006; 97: 452-458.

14. Kumaran A, Karunakaran R J: Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem., 2006 ; 97: 109-114.

15. Oyaizu M: Studies on product on browning reaction prepared from glucose amine. Jpn. J. Nutr., 1986; 44: 307-315.

16. Blois M S: Antioxidant determination by the use of of a stable free radical. Nature, 1958 ; 181: 1199-1200.

17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C: Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med., 1999; 26: 1231-1237.

18. Ali H, Houghton PJ, Soumyanath A: α- Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to phyllanthus amarus. J Ethnopharmacol, 2006; 107: 449-55.

19. Kim JS, Hyun TK, Kim MJ: The inhibitory effects of ethanol extracts from sorghum, foxtail millet and proso millet on 𝛼 -glucosidase and 𝛼 -amylase activities. Food Chem., 2011; 124: 1647–51.

20. Shai LJ., Masoko P, Mokgotho MP, Magano SR, Mogale MA, Boaduo N, Eloff JN. Yeast alpha glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa. South African J. Bot., 2010; 76: 465 470.

21. Chandrashekharaiah KS, Menaka N Bolaki, Gupta Shivani Sanjay, Aanchal Bathija, Murthy VK, Narayanaswamy M, Swamy NR: Anti-oxidant and Anti-hyperglycemic Properties of Methanolic Extracts of Medicinal Plants. Biosci., Biotech. Res. Asia, 2013; 10(2): 607-612.

22. Florence O J, Adeolu A A, Anthony J A: Comparison of the nutritive value, antioxidant and antibacterial activities of Sonchus asper and Sonchus oleraceus Rec. Nat. Prod., 2011; 5 (1): 29-42.

23. Pourmorad F, Hosseinimehr S J, Shahabimajd N: Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr. J. Biotechnol., 2006; 5(11): 1142-1145.

24. Koleva I I, Van Beek TA, Linssen J P H, Groot A D, Evstatieva LN: Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem. Anal., 2002; 13: 8-17.

25. Mai TT, Thu NN, Tien PG, Van Chuyen N: Alpha-glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents. J Nutr Sci Vitaminol (Tokyo), 2007, 53: 267–76.

26. Ramkumar KM., Thayumanavan B, Palvannan T, Rajaguru P. Inhibitory effect of Gymnema Montanum leaves on α-glucosidase activity and α-amylase activity and their relationship with polyphenolic content. Medicinal Chemistry Research, 2010; 19(8): 948-961.

27. Manikandan R, Vijaya A, Muthumani GD: Phytochemical and in vitro anti-diabetic activity of methanolic extract of Psidium guajava leaves. International Journal of Current Microbiology and Applied Sciences, 2013; 2(2):15-19.

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