Research Article | Volume 13, Supplement 1, July, 2025

Extraction, modification, and characterization of starch from two barley (Hordeum vulgare L.) cultivars

Shweta Dhiman,  Krishan Kumar,  Naseer Ahmed,  Tajendra Pal Singh,  Divya Chauhan,  Sachin Kumar,  Paras Sharma,  Ajar Nath Yadav   

Shweta Dhiman1, Krishan Kumar2, Naseer Ahmed1, Tajendra Pal Singh1, Divya Chauhan1, Sachin Kumar1, Paras Sharma3, Ajar Nath Yadav4

1Department of Food Technology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour, India.

2Department of Food Technology, Rajiv Gandhi University, Doimukh, Arunachal Pradesh, India.

3Department of Food Technology, Mizoram University, Aizawl, India.

4Department of Biotechnology, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib, Sirmour, India.

5Research and Innovation Cell, Rayat Bahra University, Mohali-140301, Punjab, India. 

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Published:  Jul 03, 2025

DOI: 10.7324/JABB.2025.214506
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Abstract

Barley (Hordeum vulgare L.) is ranked fourth amongst the cereals after maize, rice, and wheat and is mostly used for malting and animal feed. The use of barley in processed food products has gained popularity in recent years. The majority of the kernel or more than 70% of its dry weight is made up of starch. The native starch is unstable to a wide range of conditions such as temperature, pH, shear forces, and so on, and thus, limits its usage to a wide range of food and non-food industrial applications. The objective of this study was to modify the starch isolated from two cultivars of barley, i.e., PL-751 and PL-830 using physical (annealing) and enzymatic (amylase) methods and to investigate the differences in functional properties of native and modified starch. The functional properties such as syneresis, light transmittance, swelling, and solubility index changed significantly in modified starches as compared to native starch. The syneresis of annealed starch for PL-751 and PL-830 increased from 85.5%–96.67% and 81.0%–94.50%, respectively. The syneresis values of amylase-treated starch for PL-830 and PL-751 at enzymatic concentration of 7.5 U increased from 86.5%–92.0% and 84.5%–89.83%, respectively. Similarly, the values increased from 88.17%–91.83% and 79.5%–87.5% for PL-830 and PL-751 at 15 U enzymatic concentration, respectively. The light transmittance of annealed starch for both PL-830 and PL-751 decreased significantly (p ≤ 0.05) during storage for 5 days. The swelling and solubility index also increased significantly (p ≤ 0.05) with an increase in temperature both in native as well as modified starch. However, a significant (p ≤ 0.05) drop in swelling and solubility index of an annealed and enzyme-modified starch was observed as compared to native starch. Starch modification is a dynamic field with vast potential for future applications in both the food and non-food industries. The physical and enzymatic modification in starch enhanced the thermal stability, swelling power, and solubility index of the starch with reduced syneresis characteristics resulting in stable gels and a better shelf life. With the help of modification techniques, minimally processed foods can be developed with an increase in consumer demand. Modified starch helps in improving the shelf life of food products by enhancing moisture retention, and inhibiting retrogradation, and thus, the texture and freshness of the product are maintained for a longer period of time.


Keyword:     Amylase annealed starch barley functional properties modified starch starch

Citation:

Dhiman S, Kumar K, Ahmed N, Singh TP, Chauhan D, Kumar S, Sharma P, Yadav AN. Extraction, modification, and characterization of starch from two barley (Hordeum vulgare L.) cultivars. J Appl Biol Biotech. 2025;13(Suppl 1): 76–85. http://doi.org/10.7324/JABB.2025.214506

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.
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Reference

1. Alcázar-Alay SC, Meireles MAA. Physicochemical properties, modifications and applications of starches from different botanical sources. Food Sci Technol 2015;35:215-36. https://doi.org/10.1590/1678-457X.6749

2. Shevkani K, Katyal M, Singh NJFCA. A comparative review of protein and starch characteristics and end-use quality of soft and hard wheat. Food Chem Adv 2024;4(2):100613. https://doi.org/10.1016/j.focha.2024.100613

3. FAO. Food and Agriculture Organization of the United Nations. FAO, Rome, Italy. Available via http://faostat.fao.org 2018

4. Quinde Z, Ullrich S, Baik BK. Genotypic variation in colour and discoloration potential of barley-based food products. Cereal Chem 2004;81:752-8. https://doi.org/10.1094/CCHEM.2004.81.6.752

5. Santana ÁL, Meireles MAA. New starches are the trend for industry applications: a review. Food Public Health 2014;4:229-41. https://doi.org/10.5923/j.fph.20140405.04

6. Punia S. Barley starch: structure, properties and in vitro digestibility-a review. Int J Biol Macromol 2020;155:868-75. https://doi.org/10.1016/j.ijbiomac.2019.11.219

7. Siroha A, Sandhu K, Punia S. Impact of octenyl succinic anhydride on rheological properties of sorghum starch. Qual Assur Saf Crop 2019;11:221-9. https://doi.org/10.3920/QAS2018.1379

8. Deka D, Sit N. Dual modification of taro starch by microwave and other heat moisture treatments. Int J Biol Macromol 2016;92:416-22. https://doi.org/10.1016/j.ijbiomac.2016.07.040

9. Punia S. Barley starch modifications: physical, chemical and enzymatic-a review. Int J Biol Macromol 2020;144:578-85. https://doi.org/10.1016/j.ijbiomac.2019.12.088

10. Santelia D Zeeman SC. Progress in Arabidopsis starch research and potential biotechnological applications. Curr Opin Biotechnol 2011;22:271-80. https://doi.org/10.1016/j.copbio.2010.11.014

11. Watson M, Isaac RA. Analytical instruments for soil and plant analysis. Soil Test Plant Anal 1990;3:691-740. https://doi.org/10.2136/sssabookser3.3ed.c26

12. Fonseca LM, El Halal SLM, Dias ARG, da Rosa Zavareze E. Physical modification of starch by heat-moisture treatment and annealing and their applications: a review. Carbohydr Polym 2021;274:118665. https://doi.org/10.1016/j.carbpol.2021.118665

13. Xu M, Saleh AS, Gong B, Li B, Jing L, Gou M, et al. The effect of repeated versus continuous annealing on structural, physicochemical, and digestive properties of potato starch. Food Res Int 2018;111:324-33. https://doi.org/10.1016/j.foodres.2018.05.052

14. Waduge R, Hoover R, Vasanthan T, Gao J, Li J. Effect of annealing on the structure and physicochemical properties of barley starches of varying amylose content. Food Res Int 2006;39:59-77. https://doi.org/10.1016/j.foodres.2005.05.008

15. Park SH, Na Y, Kim J, Kang SD, Park KH. Properties and applications of starch modifying enzymes for use in the baking industry. Food Sci Biotechnol 2018;27:299-312. https://doi.org/10.1007/s10068-017-0261-5

16. Negi A, Barthwal R, Kathuria D, Singh NJFB. Enzymatic advances in starch modification: creating functional derivatives and exploring applications. Food Biosci 2024;62:105074. https://doi.org/10.1016/j.fbio.2024.105074

17. Hutabarat DJC, Stevensen J. Physicochemical properties of enzymatically modified starch: a review. IOP Conf Series: Earth Environ Sci 2023;1169:012093. https://doi.org/10.1088/1755-1315/1169/1/012093

18. Wang S, Li C, Copeland L, Niu Q, Wang S. Starch retrogradation: a comprehensive review. Compr Rev Food Sci Food Saf 2015;14:568-85. https://doi.org/10.1111/1541-4337.12143

19. Miao M, Xiong S, Jiang B, Jiang H, Cui SW, Zhang T. Dual-enzymatic modification of maize starch for increasing slow digestion property. Food Hydrocoll 2014;38:180-5. https://doi.org/10.1016/j.foodhyd.2013.12.006

20. Mesquita CDB, Leonel M, Mischan MM. Effects of processing on physical properties of extruded snacks with blends of sour cassava starch and flaxseed flour. Food Sci Technol 2013;33:404-10. https://doi.org/10.1590/S0101-20612013005000073

21. Bajaj R, Trehan S, Singh N, Kaur A, Passi VJSS. Properties of iron fortified starches and their chapatti making potential. Starch Stärke 2023;75:2200288. https://doi.org/10.1002/star.202200288

22. Nurmilah S, Subroto E. Chemical modification of starch for the production of resistant starch type-4 (RS4): a review. Int J Eng Trends Technol 2021;69:45-50. https://doi.org/10.14445/22315381/IJETT-V69I7P206

23. Kumar R, Ghoshal G, Goyal M. Biodegradable composite films/ coatings of modified corn starch/gelatin for shelf life improvement of cucumber. J Food Sci Technol 2021;58:1227-37. https://doi.org/10.1007/s13197-020-04685-y

24. Ranganna S, Handbook of analysis and quality control for fruit and vegetable products. Tata McGraw-Hill Education, New York, NY, 1986.

25. Vizhi VK, Many JN. Study on estimation, extraction and analysis of barley beta-glucan. Int J Sci Res 2014;3:1480-4.

26. AOAC International. Official methods of analysis. AOAC International, Washington, DC, p 684, 1990.

27. Huang S, Shiau C, Liu T, Chu C, Hwang DF. Effects of rice bran on sensory and physico-chemical properties of emulsified pork meatballs. Meat Sci 2005;70:613-9. https://doi.org/10.1016/j.meatsci.2005.02.009

28. Jones D, Chinnaswamy R, Tan Y, Hanna M. Physiochemical properties of ready-to-eat breakfast cereals. Cereal Foods World 2000;45:164-8.

29. Williams PC, Nakoul H, Singh K. Relationship between cooking time and some physical characteristics in chickpeas (Cicer arietinum L.). J Sci Food Agric 1983;34:492-6. https://doi.org/10.1002/jsfa.2740340510

30. Shaikh M, Ali TM, Hasnain A. Utilization of chemically modified pearl millet starches in preparation of custards with improved cold storage stability. Int J Biol Macromol 2017;104:360-6. https://doi.org/10.1016/j.ijbiomac.2017.05.183

31. Sofi BA, Wani IA, Masoodi FA, Saba I, Muzaffar S. Effect of gamma irradiation on physicochemical properties of broad bean (Vicia faba L.) starch. LWT-Food Sci Technol 2013;54:63-72. https://doi.org/10.1016/j.lwt.2013.05.021

32. Ashwar BA, Gani A, Wani IA, Shah A, Masoodi FA, Saxena DC. Production of resistant starch from rice by dual autoclaving-retrogradation treatment: in-vitro digestibility, thermal and structural characterization. Food Hydrocoll 2016;56:108-17. https://doi.org/10.1016/j.foodhyd.2015.12.004

33. Pérez EE, Lares M, González ZM. Characterization of starch isolated from white and dark sorghum. Starch-Stärke 1997;49:103-6. https://doi.org/10.1002/star.19970490305

34. Devi R, Sit N. Effect of single and dual steps annealing in combination with hydroxypropylation on physicochemical, functional and rheological properties of barley starch. Int J Biol Macromol 2019;129:1006-14. https://doi.org/10.1016/j.ijbiomac.2019.02.104

35. Zhai Y, Li X, Bai Y, Jin Z, Svensson BJFH. Maltogenic α-amylase hydrolysis of wheat starch granules: mechanism and relation to starch retrogradation. Food Hydrocoll 2022;124:107256. https://doi.org/10.1016/j.foodhyd.2021.107256

36. Bai YP, Zhou HM, Zhu KR, Li Q. Effect of thermal treatment on the physicochemical, ultrastructural and nutritional characteristics of whole grain highland barley. Food Chem 2021;346:128657. https://doi.org/10.1016/j.foodchem.2020.128657

37. Obadi M, Sun J, Xu B. Highland barley: chemical composition, bioactive compounds, health effects, and applications. Food Res Int 2021;140:110065. https://doi.org/10.1016/j.foodres.2020.110065

38. Alijošius S, Švirmickas GJ, Kliševi?i?t? V, Gružauskas R, Šašyt? V, Racevi?i?t?-Stupelien? A, et al. The chemical composition of different barley varieties grown in Lithuania. Vet Ir Zootech 2016;73:9-13. https://doi.org/10.13080/z-a.2016.103.035

39. Storsley J, Izydorczyk M, You S, Biliaderis C, Rossnagel B. Structure and physicochemical properties of β-glucans and arabinoxylans isolated from hull-less barley. Food Hydrocoll 2003;17:831-44. https://doi.org/10.1016/S0268-005X(03)00104-8

40. Chen X, Shao S, Chen M, Hou C, Yu X, Xiong F. Morphology and physicochemical properties of starch from waxy and non-waxy barley. Starch-Stärke 2020;72:1900206. https://doi.org/10.1002/star.201900206

41. Šterna V, Zute S, J?kobsone I. Grain composition and functional ingredients of barley varieties created in Latvia. In Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences, 2015. https://doi.org/10.1515/prolas-2015-0023

42. Panizo-Casado M, Déniz-Expósito P, Rodríguez-Galdón B, Afonso- Morales D, Ríos-Mesa D, Díaz-Romero C, et al. The chemical composition of barley grain (Hordeum vulgare L.) landraces from the Canary Islands. J Food Sci 2020;85:1725-34. https://doi.org/10.1111/1750-3841.15144

43. Singh N, Kaur L, Sandhu KS, Kaur J, Nishinari K. Relationships between physicochemical, morphological, thermal, rheological properties of rice starches. Food Hydrocoll 2006;20:532-42. https://doi.org/10.1016/j.foodhyd.2005.05.003

44. Gupta M, Bawa AS, Semwal AD. Morphological, thermal, pasting, and rheological properties of barley starch and their blends. Int J Food Prop 2009;12:587-604. https://doi.org/10.1080/10942910801947763

45. Li W, Xiao X, Zhang W, Zheng J, Luo Q, Ouyang S, et al. Compositional, morphological, structural and physicochemical properties of starches from seven naked barley cultivars grown in China. Food Res Int 2014;58:7-14. https://doi.org/10.1016/j.foodres.2014.01.053

46. Waleed AA, Mushtaq BS, Mahdi AA, Al-Maqtari QA, Abduqader AA, Ahmed A, et al. Molecular structure, morphological, and physicochemical properties of highlands barley starch as affected by natural fermentation. Food Chem 2021;356:129665. https://doi.org/10.1016/j.foodchem.2021.129665

47. Ali TM, Hasnain A. Effect of annealing on morphological and functional properties of Pakistani white sorghum (Sorghum bicolor) starch. J Basic Appl Sci 2016;12:358-64. https://doi.org/10.6000/1927-5129.2016.12.55

48. Mendes MP, Demiate IM, Monteiro ARG. Effect of individual and combined physical treatments on the properties of corn starch. Acta Sci Technol 2018;40:1-7. https://doi.org/10.4025/actascitechnol.v40i1.35118

49. Sudheesh C, Sunooj KV, Alom M, Kumar S, Sajeevkumar VA, George J. Effect of dual modification with annealing, heat moisture treatment and cross-linking on the physico-chemical, rheological and in vitro digestibility of underutilised kithul (Caryota urens) starch. J Food Meas Charact 2020;14:1557-67. https://doi.org/10.1007/s11694-020-00404-5

50. Ji Y. Effect of annealing on the functional properties of corn starch/ corn oil/lysine blends. Int J Biol Macromol 2020;144:553-9. https://doi.org/10.1016/j.ijbiomac.2019.12.122

51. Zhong Y, Herburger K, Kirkensgaard JJK, Khakimov B, Hansen AR, Blennow AJFH. Sequential maltogenic α-amylase and branching enzyme treatment to modify granular corn starch. Food Hydrocoll 2021;120:106904. https://doi.org/10.1016/j.foodhyd.2021.106904

52. Balasubramanian S, Sharma R, Kaur J, Bhardwaj N. Characterization of modified pearl millet (Pennisetum typhoides) starch. J Food Sci Technol 2014;51:294-300. https://doi.org/10.1007/s13197-011-0490-1

53. Keeratiburana T, Hansen AR, Soontaranon S, Blennow A, Tongta S. Porous high amylose rice starch modified by amyloglucosidase and maltogenic α-amylase. Carbohydr Polym 2020;230:115611. https://doi.org/10.1016/j.carbpol.2019.115611

54. Ji N, Ge S, Li M, Wang Y, Xiong L, Qiu L, et al. Effect of annealing on the structural and physicochemical properties of waxy rice starch nanoparticles: effect of annealing on the properties of starch nanoparticles. Food Chem 2019;286:17-21. https://doi.org/10.1016/j.foodchem.2019.01.205

55. Ji N, Li X, Qiu C, Li G, Sun Q, Xiong L. Effects of heat moisture treatment on the physicochemical properties of starch nanoparticles. Carbohydre Polym 2015;117:605-9. https://doi.org/10.1016/j.carbpol.2014.10.005

56. Khatoon S, Sreerama Y, Raghavendra D, Bhattacharya S Bhat K. Properties of enzyme modified corn, rice and tapioca starches. Food Res Int 2009;42:1426-33. https://doi.org/10.1016/j.foodres.2009.07.025

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