Research Article | Volume: 4, Issue: 3, May-June, 2016

Antimicrobial Activity Screening of Marine Bacteria Isolated from the Machilipatnam Sea Coast of Andhra Pradesh, India

K. Bala Chandra V. Umamaheswara Rao Subhaswaraj Pattnaik Siddhardha Busi   

Open Access   

Published:  Jun 21, 2016

DOI: 10.7324/JABB.2016.40303

Bacterial colonies were obtained from water samples collected from sea-coast of Machilipatnam, Andhra Pradesh in South India. The isolates were cultured, named as M1, M2, M3 etc., and screened for antibacterial action. Among the 40 representative isolates obtained, three isolates showed substantial antibacterial activity with more than 10 mm of zone of inhibition against two test bacteria used in preliminary screening. These three isolates marked as M20, M22 and M23 showed most outstanding results against all the test bacteria used in further testing of antibacterial activity. The isolate M20 showed the highest activity with a zone of inhibition of 1a6mm against Serratia marcescens, 14 mm against E.coli and Shigella, and with 12 mm against Salmonella paratyphi and Bacillus cereus. The isolates M20, M22 and M23 were analyzed for their morphological, biochemical and physiological characteristics and tentatively identified as Alcaligenes sp. (M20), Bacillus sp. (M22) and Bacillus sp. (M23) from the performed biochemical tests.

A corrigendum is published for this article, read the linked corrigendum.

Keyword:     Crude extractsAntibacterial activitySecondary metabolitesCarbon source utilization.


Chandra KB, Rao VU, Pattnaik S, Busi S. Antimicrobial Activity Screening of Marine Bacteria Isolated from the Machilipatnam Sea Coast of Andhra Pradesh, India. J App Biol Biotech. 2016; 4 (03): 015-019. DOI: 10.7324/JABB.2016.40303

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text

1. Demain AL. Pharmacuetically active secondary metabolites of micro organisms. Applied Microbiology and Biotechnolology. 1999; 52: 455-463.

2. Janos Berdy. New trends in the research of bioactive microbial metabolites. In chemistry and biotechnology of biologically active natural products. 4th int. conf., Budapest. 1987, p. 269-291.

3. Janos Berdy. Bioactive microbial metabolites - A personal view. Journal of Antibiotics. 2005; 58(1): 1-26.

4. Woodruff HB. Natural products from micro organisms. Science. 1980; 208(4449): 1225-1229.

5. Fernando Pelaez. The historical delivery of antibiotics from microbial natural products – can history repeat? Biochemical Pharmacology. 2006; 71(7): 981-990.

6. Janos Berdy. Screening, classification and identification of microbial products. In discovery and isolation of microbial products, Ed. Verral MS. Eills Horwood, Chichester. 1980, p. 9-31.

7. Chavasse DC and Yap HH. Chemical Methods for the Control of vectors and pests of public health importance. WHO/CDT/WHOPES/97.2.27, Geneva, Switzerland,1997.

8. Omena MC, Navarro DMAF, Paula de JE, Luna JS, Ferreira de Lima MR, Sant’Ana AEG. Larvicidal activities against Aedes aegypticus of some brazillian medicinal plants. Bioresources Technology. 2007; 98: 2549-2556.

9. World Health Organisation. Instructions for determining susceptibility or resistance of mosquito larvae to insecticides. WHO/VBC-81, 1981, p. 807.

10. Perez C, Pauli M, Bazerque P. An Antibiotic assay by the agar well diffusion method. Acta Biologiae et Medecine Experimentaalis. 1990; 15: 113-115.

11. Lapage S.P. Biochemical test for identification of medical bacteria. Journal of Clinical Pathology. 1976; 29.

12. MacFaddin JF. Biochemical tests for identification of medical bacteria, 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA, 2000.

13. Patricia H Clarke. Hydrogen Sulphide Production by Bacteria. Microbiology. 1953; 8: 397-407.

14. Jay L. Garland and Aaron L. Mills. Classification and Characterization of Heterotrophic Microbial Communities on the Basis of Patterns of Community-Level Sole-Carbon-Source Utilization. Applied and Environmental Microbiology. 1991; 57(8): 2351-2359.

15. Mahon CR, Lehman DC, Manuselis G. . Textbook of diagnostic microbiology, 4th ed. W. B. Saunders Co., Philadelphia, PA, 2011.

16. Cleidson Valgas; Simone Machado de Souza; Elza F A Smânia; Artur Smânia Jr. Screening methods to determine antibacterial activity of natural products. Brazilian Journal of Microbiology. 2007; 38: 369-380.

17. Alejandra Cetina, Adriana Matos, Gabriel Garma, Helena Barba, Rosario Vazquez, Armando Zepeda-Rodriguez, David Jay, Víctor Monteon, Ruth Lopez-A. Antimicrobial activity of marine bacteria isolated from Gulf of Mexico. Revista Peruana de Biologia. 2010; 17(2): 231–236.

18. Florence Depardieu, Isabelle Podglajen, Roland Leclercq, Ekkehard Collatz, Patrice Courvalin. Modes and modulations of antibiotic resistance gene expression. Clinical Microbiology Review. 2007; 20(1): 79-114.

19. Kin S Lam Discovery of novel metabolites from marine actinomycetes. Current opinion in microbiology.Science Direct 2006: 245-251.

Article Metrics
134 Views 77 Downloads 211 Total



Related Search

By author names

Similar Articles