Author's Guidelines

Journal of Applied Biology & Biotechnology follows ICMJE: Uniform Requirements for Manuscripts Submitted to Biomedical Journals. Please visit for details regarding editorial and peer-review policies.

Preparation of Manuscript 

The manuscript should be typed in English, double-spaced on standard A4 sized paper (8.5" x 11") with 1" margins on all sides. Times New Roman font 12 pt should be used. The fonts used in the text, as well as graphics, should be restricted to Times New Roman, Symbol and Zapf Dingbats.

1. Title page

The title page should contain a clear, concise, and informative title of the article followed by the names and affiliations of the authors. The affiliation should comprise the department, institution (usually university or company), city, and state (or nation) and should be typed as a footnote to the author's name. The Corresponding Author must indicate his or her complete mailing address, office/cellular phone number, fax number, and email address at the lower left of the Title Page. The authors should ensure that their manuscript(s) must fall within aims and scope. Authors are required to indicate the specific subject area, mentioned in aims and scope, on the top of the title page. For example; Microbiology, Biotechnology or Structural biology, etc.

2. Article Structure

Articles should be divided into clearly defined and numbered sections. Subsections should also be numbered for example: 1.1 (then 1.1.1, 1.1.2, ...), 1.2, etc.

2.1 Abstract

The abstract should not more than 200 words and should provide brief details regarding objectives, material, and methods, Results, and Conclusions. Reviews and mini reviews also require an abstract. The abstract should not be structured, it should be a single paragraph without subheadings. The abstract must be followed by four-six keywords.

2.2 Introduction

This should be brief and indicates the aim of the study and the essential background information. The introduction should clearly state the hypothesis or purpose statement, how and why the purpose or hypothesis was developed and why the author deems it important.

2.3 Material and methods

Please provide concise but complete information about the material and the analytical, statistical and experimental procedures used. This part should be as clear as possible to enable other scientists to repeat the research presented. The use of subheadings to divide the text is encouraged. Primary headings should be in BOLD CAPITAL LETTERS. Secondary or subheadings should be in Bold sentence case. Third level subheadings should be in Italicized sentence Case. In the case of animal experiments, authors must give the details of ethical approval.

2.4 Result and Discussion

Data acquired from the research with the appropriate statistical analysis described in the methods section should be included in this section. In this part, the same data/ information given in a table must not be repeated in a figure or vice versa. Tables and Figures should be self-explanatory and it is not acceptable to repeat extensively the numerals from tables into text and give lengthy and unnecessary explanations of the Tables and Figures. The discussion should relate the results to the current understanding of the scientific problems being investigated in the field.

2.5 Conclusion

This section should clearly explain the main conclusions of the work highlighting its importance and relevance.

2.6 References

References should be numbered consecutively throughout the article, beginning with [1] for the first-cited reference. References should be listed at the end of the paper in the order in which they appear in the text.

References should be cited in the text by numerals in square bracket. For example;

…… Cell cycle arrest and apoptosis induction are targeted in the strategy of cancer therapy [1]. Apoptosis, or programmed cell death, is a multi-step process that is important to eliminate damaged or abnormal cells [2]. Chemopreventive agents comprise a diverse group of compounds with different mechanisms of action, but, their ultimate ability to induce apoptosis may represent a unifying concept for the mechanism of chemoprevention [3]……

The authors should ensure that every reference cited in the text is also present in the reference list (and vice versa).

2.6.1 Journal article

Ramesh CK, Rehman A, Prabhakar BT, Vijay Avin BR, Aditya Rao SJ. Antioxidant potential in sprouts vs. seeds of Vigna radiata and Macrotyloma uniflorum. Journal of Applied Pharmaceutical Science. 2011; 1(3):99-103.

2.6.2 Reference to a book:

Strunk Jr W, White EB. The elements of style. 4th ed. New York: Longman; 2000.

2.6.3 Reference to a chapter in an edited book:

Mettam GR, Adams LB. How to prepare an electronic version of your article. In: Jones BS, Smith RZ, editors. Introduction to the electronic age, New York: E-Publishing Inc; 2009, p. 281–304.

2.6.4 Electronic Material

Anderson SC, Poulsen KB. Anderson's electronic atlas of hematology [CD-ROM]. Philadelphia: Lippincott Williams & Wilkins; 2002.

Journal article on the Internet:

Abood S. Quality improvement initiative in nursing homes: the ANA acts in an advisory role. Am J Nurs [Internet]. 2002 Jun [cited 2002 Aug 12];102(6):[about 1 p.]. Available from: http://www.nursingworld. org/AJN/2002/june/Wawatch.htmArticle

Article with document number in place of traditional pagination:

Williams JS, Brown SM, Conlin PR. Videos in clinical medicine. Blood-pressure measurement. N Engl J Med. 2009 Jan 29;360(5):e6.

Article with a Digital Object Identifier (DOI):

Zhang M, Holman CD, Price SD, Sanfilippo FM, Preen DB, Bulsara MK. Comorbidity and repeat admission to hospital for adverse drug reactions in older adults: retrospective cohort study. BMJ. 2009 Jan 7;338:a2752. doi: 10.1136/bmj.a2752.

Monograph on the Internet:

Foley KM, Gelband H, editors. Improving palliative care for cancer [Internet]. Washington: National Academy Press; 2001 [cited 2002 Jul 9]. Available from:

Homepage/Web site: [Internet]. New York: Association of Cancer Online Resources, Inc.; c2000-01 [updated 2002 May 16; cited 2002 Jul 9]. Available from

Please visit; for more details about the reference style.


3. Authorship, Acknowledgements, and Conflicts of interest 

3.1 Authorship

All the authors in a manuscript are equally responsible for the technical information communicated to the journal. Thus, it is necessary that all authors must read and approve the final version of the manuscript before submitting it to the journal. All named authors must have made an active contribution to the conception and design and/or analysis and interpretation of the data and/or the drafting of the paper and all must have critically reviewed its content and have approved the final version submitted for publication. Participation solely in the acquisition of funding or the collection of data does not justify authorship.

JABB follows the definition of authorship given by the International Committee of Medical Journal Editors (ICMJE). The ICMJE recommends that authorship be based on the following 4 criteria:

  • Substantial contributions to the conception or design of the work; or the acquisition, analysis, or interpretation of data for the work; AND
  • Drafting the work or revising it critically for important intellectual content; AND
  • Final approval of the version to be published; AND
  • Agreement to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.


Authors should meet all the four conditions.  Please click here to download the Authorship Contribution Form, authors are required to upload the duly filled and signed form under the additional files section, during manuscript submission. They can also send it by email to In addition to that, all the manuscripts should contain Authors’ Contributions statement immediately before the Acknowledgments section. 

3.2 Acknowledgements

All acknowledgments should be included at the very end of the paper before the references and may include supporting grants, presentations, and so forth. Contributors who do not qualify as authors should be mentioned under Acknowledgements.

3.3 Conflicts of interest

All the authors must disclose the possible conflicts of interest/Competing Interests they may have with publication of the manuscript or an institution or product that is mentioned in the manuscript and/or is important to the outcome of the study presented. Authors should also disclose conflict of interest with products that compete with those mentioned in their manuscript. The Conflict of Interest statement should list each author separately by name. The ICMJE has developed a Disclosure Form to facilitate and standardize authors’ disclosures, please click this link to download the ICMJE COI form. Authors are required to upload the duly filled and signed form under the additional files section, during manuscript submission, or send it by email to the Please follow the link to read the detailed ICMJE recommendations for disclosure of financial and non-financial relationships and activities, and conflicts of interest. 

3.4 Funding sources 

The authors must declare all sources of funding received for the research submitted to the journal. Authors should give the name of granting agencies and grant numbers, along with a short description of each funder’s role.  Only the funding related to the submitted work should be mentioned and authors are NOT required to provide the complete list of every single grant that supports them. 


4. Tables & Figures

Tables and figures should not be embedded in the text but should be included at the end of the manuscript on separate pages. Tables should be created with a word processor and cited consecutively in the text. To ensure the highest print quality, your figures must be submitted in JPEG/JPG/TIF format with a minimum of 300 dpi or higher resolutions. Captions/legends will be placed below figures and adjusted to 10 font size.


5. Ethical Approvals

Patient Rights

Patients have a right to privacy thus; all the studies should be conducted with informed consent. No identifying information should be published in written descriptions, photographs, and pedigrees unless the information is essential for scientific purposes and the patient (or parent or guardian) gives written informed consent for publication. When informed consent has been obtained, it should be indicated in the article. Investigations with human subjects must follow the ethical standards formulated in the Helsinki Declaration of 1964, revised in 2000. All the experiments should be approved by the institutional human experimentation committee or equivalent.  

Animal Rights

Animal experiments must follow the ethical standards for the care and use of laboratory animals. The article involved the animal experiments must seek approval from the appropriate Ethical Committee in accordance with "Principles of Laboratory Animal Care" (NIH publication no. 85-23, revised 1985). 

Clinical trial registry

We advise the authors to register the clinical trials with a clinical trial registry that allows free online access to the public. Registration in the following trial registers is acceptable:;  and


6. Abbreviations

Standard abbreviations should be used throughout the manuscript. All nonstandard abbreviations should be kept to a minimum and must be defined in the text following their first use.


7. Article Processing Charges (APCs)

The authors are required to pay Rs. 7500/- (Indian Authors) or USD 200 (foreign authors) for APCs upon acceptance of the manuscript. APCs are applicable only to the accepted articles. 

Note: These charges are calculated for a standard length article with 20 pages in MS-Word (1-inch margin on each side and two-line space). Additional page charges 250 INR per page (Indian authors) or 7 USD per page (foreign authors) will be applicable for the articles beyond this limit.