Research Article | Volume: 5, Issue: 4, July-August, 2017

Practiced Gram negative bacteria from dyeing industry effluents snub metal toxicity to survive

Channarayapatna-Ramesh Sunilkumar Lobo Rachel Gurulingaiah Bhavya Kujur Swati R. Samaga Sridhar J. Samanth Kumar K. Ramachandra Kini H. S. Prakash Nagaraja Geetha   

Open Access   

Published:  Aug 14, 2017

DOI: 10.7324/JABB.2017.50406

Isolation and characterization of metal-tolerant bacteria from dyeing industry effluent was the prime drive of the present investigation. Results yielded during screening and isolation, noted the dominance of bacteria verses fungi; in addition, metal tolerance ability of six out of twenty five bacterial isolates were observed. The six selected isolates were gram’s negative, and were identified as Stenotrophomonas maltophilia UOMGNS116, Pseudomonas sp. UOMGNS216, Pseudomonas stutzeri UOMGNS316, Pseudomonas stutzeri UOMGNS416, Citrobacter freundii UOMGNS516 and Achromobacter sp. UOMGNS616 by 16s rDNA analysis. This investigation also evidenced the varied resistance capabilities by Achromobacter sp. UOMGNS616 and Citrobacter freundii UOMGNS516 against chromium and lead by overproduction of external polysaccharides especially against lead.

Keyword:     Bioremediation Metal tolerant bacteria effluents Achromobacter sp Stenotrophomonas maltophilia.


Sunilkumar CR, Rachel L, Bhavya G, Swati K, Sridhar RS, Samanth Kumar J, Kini KR, Prakash HS, Geetha N. Practiced Gram negative bacteria from dyeing industry effluents snub metal toxicity to survive. J App Biol Biotech. 2017; 5 (04): 037-042.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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1. D'amore JJ, Al-Abed SR, Scheckel KG, Ryan JA. Methods for speciation of metals in soils. Journal of Environmental Quality. 2005; 34(5):1707-45.

2. Sposito G, Page AL. Cycling of metal ions in the soil environment. Metal ions in biological systems. 1984; 18:287-332.

3. Armah FA, Quansah R, Luginaah I. A systematic review of heavy metals of anthropogenic origin in environmental media and biota in the context of gold mining in Ghana. International Scholarly Research Notices. 2014.

4. Raven PH, Berg LR, Johnson GB. Environment. Saunders College Publishing. 2nd ed. New York, NY, USA; 1998.

5. Jones LHP, Jarvis SC. The fate of heavy metals. The Chemistry of Soil Processes. 1981; 593-620.

6. Reed SC, Crites RW, Middlebrooks EJ. Natural systems for waste management and treatment. 2nd ed. McGraw-Hill, Inc.; 1995.

7. Bjuhr J. Trace metals in soils irrigated with waste water in a Periurban area downstream Hanoi City, Vietnam, Seminar Paper, Institutionen for Markvetenskap, Sveriges lantbruk- ¨ Suniversitet (SLU), Uppsala, Sweden, 2007.

8. Babel S, Kurniawan TA. Cr (VI) removal from synthetic wastewater using coconut shell charcoal and commercial activated carbon modified with oxidizing agents and/or chitosan. Chemosphere. 2004; 54(7):951-967.

9. Sörme L, Lagerkvist R. Sources of heavy metals in urban wastewater in Stockholm. Science of the Total Environment. 2002; 298(1):131-45.

10. Gunatilake SK. Methods of removing heavy metals from industrial wastewater. Methods. 2015; 1(1).

11. Kirpichtchikova TA, Manceau A, Spadini L, Panfili F, Marcus MA, Jacquet T. Speciation and solubility of heavy metals in contaminated soil using X-ray microfluorescence, EXAFS spectroscopy, chemical extraction, and thermodynamic modeling. Geochimica et Cosmochimica Acta. 2006; 70(9):2163-2190.

12. Kurniawan TA, Chan GY, Lo WH, Babel S. Physico-chemical treatment techniques for wastewater laden with heavy metals. Chemical Engineering Journal. 2006; 118(1):83-98.

13. Malik A. Metal bioremediation through growing cells. Environment International. 2004; 30(2):261-78.

14. Schwarzenbach RP, Escher BI, Fenner K, Hofstetter TB, Johnson CA, Von Gunten U, Wehrli B. The challenge of micropollutants in aquatic systems. Science. 2006; 313(5790):1072-1077.

15. Boopathy R. Factors limiting bioremediation technologies. Bioresource Technology. 2000; 74(1):63-67.

16. Sunil KCR, Swati K, Bhavya G, Nandhini M, Veedashree M, Prakash HS, Kini KR, Geetha N. Streptomyces flavomacrosporus, A multi-metal tolerant potential bioremediation candidate isolated from paddy field irrigated with industrial effluents. Int. J. of Life Sciences. 2015; 3(1):9-15.

17. Vincent JM. A manual for the practical study of the root-nodule bacteria. A manual for the practical study of the root-nodule bacteria. 1970.

18. James GC, Sherman N. Microbiology: A laboratory manual. Benjamin Publishing Co. Inc, California, 1987.

19. Hankin L, Anagnostakis SL. The use of solid media for detection of enzyme production by fungi. Mycologia. 1975; 1:597-607.

20. Doyle J. DNA protocols for plants. In Molecular techniques in taxonomy. Springer Berlin Heidelberg 1991, pp. 283-293.

21. Lloyd JR. Bioremediation of metals; the application of micro-organisms that make and break minerals. Interactions. 2002; 2:M2.

22. Nancharaiah YV, Mohan SV, Lens PN. Biological and bioelectrochemical recovery of critical and scarce metals. Trends in biotechnology. 2016; 34(2):137-155.

23. Kapoor A, Viraraghavan T. Fungal biosorption - an alternative treatment option for heavy metal bearing wastewaters: a review. Bioresource technology. 1995; 53(3):195-206.

24. Zouboulis AI, Loukidou MX, Matis KA. Biosorption of toxic metals from aqueous solutions by bacteria strains isolated from metal-polluted soils. Process Biochemistry. 2004; 39(8):909-916.

25. Padan E, Bibi E, Ito M, Krulwich TA. Alkaline pH homeostasis in bacteria: new insights. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2005; 1717(2):67-88.

26. Schuldiner S, Agmon V, Brandsma J, Cohen A, Friedman E, Padan E. Induction of SOS functions by alkaline intracellular pH in Escherichia coli. Journal of Bacteriology. 1986; 168(2):936-9.

27. Maurer LM, Yohannes E, Bondurant SS, Radmacher M, Slonczewski JL. pH regulates genes for flagellar motility, catabolism, and oxidative stress in Escherichia coli K-12. Journal of Bacteriology. 2005; 187(1):304-19.

28. Rouch DA, Lee BT, Morby AP. Understanding cellular responses to toxic agents: a model for mechanism-choice in bacterial metal resistance. Journal of industrial microbiology. 1995; 14(2):132-41.

29. François F, Lombard C, Guigner JM, Soreau P, Brian-Jaisson F, Martino G, Vandervennet M, Garcia D, Molinier AL, Pignol D, Peduzzi J. Isolation and characterization of environmental bacteria capable of extracellular biosorption of mercury. Applied and Environmental Microbiology. 2012; 78(4):1097-106.

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