Research Article | Volume: 5, Issue: 3, May-June, 2017

Effect of extraction solvents on phenolic contents and antioxidant capacities of Artocarpus chaplasha and Carissa carandas fruits from Bangladesh

Gouri Dhar Sonam Akther Afrin Sultana Uchen May Mohammed Moinul Islam Mowri Dhali Dwaipayan Sikdar   

Open Access   

Published:  Jun 19, 2017

DOI: 10.7324/JABB.2017.50307
Abstract

Increased intake of fruits, due to their high antioxidant capacity, is known to reduce the risk of many diseases. The high antioxidant capacity of fruits is attributed by the presence of phenolics. The aim of the present study was to evaluate how solvents affect the extraction of phenolic and flavonoid substances and the antioxidant activity of fruit extracts of A. chaplasha and C. carandas. The fruits were extracted using water and various concentrations (50%, 70% and pure) of methanol, ethanol and acetone in water as solvent. Phenolic and flavonoid contents were measured by Folin-Ciocalteu method and aluminum trichloride method respectively. Antioxidant activity was measured by DPPH free radical scavenging assay. The efficiency of solvents in extracting phenols varied considerably for both fruits. Phenolic content and antioxidant activity was highest in 70% acetone extract and lowest in 100% acetone extract whilst flavonoid content was highest in pure ethanol extracts of both fruit. Strong correlation was found between the phenolic contents and antioxidant activities. Between the two fruits, C. carandas had higher phonolic content and antioxidant activity in all solvents suggesting the fruit could be a potential source of natural antioxidant for food and nutraceutical applications.


Keyword:     Phenolic contentsFlavonoidsAntioxidant activitySolvent effectFruits.


Citation:

Dhar G, Akther S, Sultana A, May U, Islam MM, Dhali M, Sikdar D. Effect of extraction solvents on phenolic contents and antioxidant capacities of A. chaplasha and C. carandas fruits from Bangladesh. J App Biol Biotech. 2017; 5 (03): 039-044.

Copyright: Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

HTML Full Text
Reference

1. Halliwell B. How to characterize an antioxidant- An update. Biochemical Society Symposium. 1995; 61:73–101.

2. Shi HL, Noguchi N, Niki N. Comparative study on dynamics of antioxidative action of α- tocopheryl hydroquinone, ubiquinol and α- tocopherol, against lipid peroxidation. Free Radical Biology and Medicine. 1999; 27:334–46.

3. Chanaj-kaczmarek J, Wysocki M, Karachitos A, Wojcińska M, Bartosz J, Matlawska I, Kmita H. Effect of plant extract antioxidative phenolic compounds on energetic status and viability of Saccharomyces cerevisiae cells undergoing oxidative stress. Journal of Functional Foods. 2015; 16:364-377.

4. Lobo V, Patil A, Phatak A, Chandra, N. Free radicals, antioxidant and functional foods: impact on human health. Pharmacognosy Review. 2010; 4(8):118-126.

5. Murillo E, Britton GB, Durant, AA. Antioxidant activity and polyphenol content in cultivated and wild edible fruits grown in Panama. J Pharmacy and Bioallied Sciences. 2012; 4(4):313–317.

6. Zhang, Y, Gan R, Li S, Zhou Y, Li A, Xu D, Li H. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules. 2015; 20: 21138-21156.

7. Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J of Agricultural and Food Chemistry. 1998; 46:4113–4117

8. Do QD, Angkawijaya AE, Tran-Nguen PL, Huynh LH, Soetaredjo FE, Ismadji S. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. Journal of Food and Drug Analysis. 2014; 22: 296-302.

9. Xu BJ, Chang SKC. A comparative study on phenolic profiles and antioxidative properties of legumes as affected by extraction solvents. Journal of Food Science. 2007; 72(Nr. 2):S159-S166.

10. Boulekbache-Makhlouf L, Medouni L, Medouni-Adrar S, Arkoub L, Madani K. Effect of solvents extraction on phenolic content and antioxidant activity of the byproduct of eggplant. Industrial Crops and Products. 2013; 49:668-674.

11. Hossain MA, Rahman SMM. Total phenolic, flavonoids and antioxidant activity of tropical fruit pineapple. Food Research International. 2011; 44:672-676.

12. Boeing JS, Barizão EO, Silva BC, Montanher PF, Almeida VC, Visentainer JV. Evaluation of solvent effect on the extraction of phenolic compounds and antioxidant capacities from the berries: application of principal component analysis. Chemistry Central Journal. 2014; 8:48.

13. Hossain SJ, Tsujiyama I, Takasugi M, Islam MA, Biswas RS, Aoshima H. Total phenolic content, antioxidative, anti-amylase, anti-glucosidase, and antihistamine release activities Bangladeshi fruits. Food Science and Technological Research. 2008; 14(3):261-268.

14. Aklima J, Mojumder S, Sikdar D. Total phenolic content, reducing power, antioxidative and anti-amylase activities of five Bangladeshi fruits. International Food Research Journal. 2014; 21(1):119-124.

15. Rahman MS, Al-amin M, Akter S. Artocurpas chaplasha: Establishment and initial growth performance at elevated temperature and saline stresses. Journal of forest Science. 2012; 28(1):12-18.

16. Chowdhury F, Pal S, Sharmin T, Rashid RB, Sikder MAA, Kabir S, Rahman MS, Rashid, MA. Bioactivities of Artocarpus chaplasha Roxb. and Bougainvillea spectabilis Willd. Bangladesh Pharmaceutical Journal. 2013; 16(1):63-68.

17. Ahmed T, Uddin MN, Ahmed SF, Saha A, Farhana K, Rana MS. In vitro evaluation of antioxidant potential of Artocarpus chama Buch. fruits. Journal of Applied Pharmaceutical Science. 2102; 2(10):075-080.

18. Anupama N, Modhmita G, Rajesh KS. Role of dried fruits of Carissa carandas as anti-inflammatory agents and the analysis of phytochemical constituents by GC-MS. BioMed Research International. 2014; 2014:512369.

19. Wetwitayuklung P, Charoenteeraboon J, Limmatvapirat C, Phaechamud T. Antioxidant activities of some Thai and exotic fruits cultivated in Thailand. Research Journal of Pharmaceutical Biological and Chemical Sciences. 2012; 3(1):12-21.

20. Sarma A, Sarmah P, Kashyap D, Dutta S, Mahanta M. Antioxidant activity and nutraceutical property of the fruits of an ethno-medicinal plant: Carrisa carandas L. found in Brahmaputra valley agro-climate condition. Journal of Pharmaceutical Sciences and Research. 2015; 7(2):55-57.

21. Pewlong W, Sajjabut S, Eamsiri J, Chookaew S. Evaluation of antioxidant activities, anthocyanins, total phenolic content, vitamin C content and cytotoxicity of Carissa carandas Linn. CMUJNS Special Issue on Food and Applied Bioscience. 2014; 13(1):509-517.

22. Kachhwaha P, Gehlot, HS. Changes in Phytonutrients and antioxidant properties of Cordia myxa and Carissa carandas fruit during ripening. Indian Journal of Plant Physiology. 2015; 20(1):72-78.

23. Ahmed ZU, Hassan MA, Begum ZNT, Khondker M, Kabir SMH, Ahmad M, Ahmed ATA. Encyclopedia of Flora and Fauna of Bangladesh, Dhaka: Asiatic Society of Bangladesh. (Eds), 2009; Vol. 9, p. 186-187.

24. Ahmed ZU, Begum ZNT, Hassan MA, Khondker M, Kabir SMH, Ahmad M, Ahmed ATA, Rahman AKA, Haque EU. Encyclopedia of Flora and Fauna of Bangladesh, Dhaka: Asiatic Society of Bangladesh. (Eds), 2008; Vol. 6, p. 178.

25. Ough CS, Amerine MA. Phenolic compounds. In Methods for analysis of musts and wines. New York, USA: Wiley and Sons; 1998. p. 196-221.

26. Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry. 1999; 64:555–59.

27. Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010; 15:7313-7352.

28. Khoddami A, welkes MA, Roberts TH. Techniques for analysis of plant phenolic compounds. Molecules. 2013; 18:2328-2375.

29. Sultana B, Anwar F, Ashraf M. Effect of extraction solvent/technique on the antioxidant activity of selected medicinal plant extracts. Molecules. 2009; 14:2167-2180.

30. Kchaou W, Abbès F, Blecker C, Attia H, Besbes S. Effects of extraction solvents on phenolic contents and antioxidant activities of Tunisian date varieties (Phoenix dactylifera L.). Industrial Crops and Products. 2013; 45:262– 269.

31. Wijekoon MMOJ, Bhat R, karim AA. Effect of extraction solvents on the phenolic compounds and antioxidant activities of Bunga kantan (Etlingera elatior Jack.) inflorescence. Journal of Food composition and Analysis. (2011); 24:615-619.

32. Meneses NGT, Martins S, Teixeira JA, Mussatto SI. Influence of extraction solvents on the recovery of antioxidant phenolic compounds from brewer’s spent grains. Separation and Purification Technology. 2013; 108:152-158.

33. Gul J, Sultana B, Anwar F, Naseer R, Ashraf M, Ashrafuzzaman M. Variation in antioxidant attributes at three ripening stages of guava (Psidium guajava L.) fruit from different regions of Pakistan. Molecules. 2012;17:3165-3180.

34. Miletic N, Popović B, Mitrović O, Kandić M. Phenolic content and antioxidant capacity of fruits of plum cv. ‘Stanley’ (Prunus domestica L.) as influenced by maturity stage and on-tree ripening. Australian Journal of Crop Science. 2012; 6(4):681-697.

35. Anokwuru CP, Anyasor GN, Ajibaye O, Fakoya O, Okebugwu P. Effect of extraction solvents on phenolic, flavonoid and antioxidant activities of three Nigerian Medicinal Plants. Nature and Science. 2011; 9(7):53-61.

36. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology. 2011; 48(4):412–422.

Article Metrics
225 Views 132 Downloads 357 Total

Year

Month

Related Search

By author names

Similar Articles